Cytochrome P450 monooxygenase (CYP)

Tlhaloso e Khutšoanyane:

Mabapi le CYP

ES-CYPs: Sehlopha sa li-enzyme tsa redox tse nang le mercaptan-heme e le setsi se sebetsang sa ho susumetsa phetisetso ea oksijene.Ke li-oksijene tsa ho qetela 'me li hloka heme ho kenya oksijene tšebetsong.

Cytochrome P450 monooxygenase e etsa hore oxidation, epoxidation le hydroxylation ea alkyl, hydroxylation le oxidation ea ammonia, oxidation ea sebabole, dealkylation (oksijene, sebabole, ammonia), oxidative dehydrogenation, deamination le dehalogenation.
Ho na le mefuta e 8 ea lihlahisoa tsa cytochrome P450 monooxygenase (Palo e le ES-CYP-101～ES-CYP-108) e ntlafalitsoeng ke SyncoZymes.

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E-mail:lchen@syncozymes.com

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Lintlha tsa Sehlahisoa

Li-tag tsa Sehlahisoa

Mabapi le CYP:

Mofuta oa karabelo ea Catalytic:

Lintlha tsa Sehlahisoa:

Li-enzyme	Skrine Kit (SynKit)	Tlhaloso
Phofo ea enzyme	ES-CYP-101~ ES-CYP-108	sehlopha sa 8 Cytochrome P450 Monooxygenases, 50 mg ntho e 'ngoe le e' ngoe 8 lintho * 50mg / ntho, kapa bongata bo bong
Skrine Kit (SynKit)	ES-CYP-800	sehlopha sa 8 Cytochrome P450 Monooxygenases, 1mg ntho e 'ngoe le e' ngoe ea 8 * 1mg / ntho

Melemo:

★ Maqhubu a pharalletseng a substrate.
★ Phetoho e phahameng.
★ Lihlahisoa tse fokolang.
★ Maemo a bonolo a karabelo.
★ Ho baballa tikoloho.

Litaelo tsa tšebeliso:

➢ Ka tloaelo, sistimi ea karabelo e tlameha ho kenyelletsa substrate, tharollo ea buffer (The optimum reaction pH of enzyme), coenzyme (NAD(H) kapa NADP(H)), coenzyme regeneration system (mohlala, glucose le glucose dehydrogenase) le ES-CYP.coenzyme le coenzyme regeneration sisteme e ka nkeloa sebaka ke hydrogen peroxide tsamaisong e sa fellang ea karabelo.
➢ Mefuta eohle ea ES-CYPs e tsamaellanang le maemo a fapaneng a karabelo e nepahetseng e lokela ho ithutoa ka bonngoe.
➢ Substrate kapa sehlahisoa se nang le matla a mangata se ka sitisa tšebetso ea ES-CYP.Leha ho le joalo, inhibition e ka imoloha ka ho eketsoa ha sehlopha sa substrate.

Mehlala ea Tšebeliso:

Mohlala 1⁽¹⁾:

Mohlala 2⁽²⁾:

Mohlala 3⁽³⁾:

Mohlala 4⁽⁴⁾:

Polokelo:

Boloka lilemo tse 2 ka tlase ho -20 ℃.

Tlhokomeliso:

Le ka mohla u se ke ua kopana le maemo a feteletseng a kang: mocheso o phahameng, pH e phahameng / tlase le solvent e phahameng ea organic.

Litšupiso:

1. Zaretzki J, Matlock M, le Swamidass S JJ Chem.Inf.Mohlala, 2013, 53, 3373-3383.
2. Gannett P M., Kabulski J, Perez F A., e tal.J. Am.Chem.Soc., 2006, 128 (26), 8374-8375.
3. Cryle M J., Matovic N J., le De Voss J J. Org.Lett., 2003, 5 (18), 3341–3344.
4. Kawauchi, H., Sasaki, J., Adachi, T., e tal.Biochim.Lithuto tsa bophelo.Acta, 1994, 1219, 179.
5. Yasutake, Y., Fujii, Y.;Cheon, WK e tal.Acta Crystallog.2009, 65, 372.