Cytochrome P450 monooxygenase (CYP)

Tlhaloso e Khutšoanyane:

Mabapi le CYP

ES-CYPs: Sehlopha sa li-enzyme tsa redox tse nang le mercaptan-heme e le setsi se sebetsang sa ho susumetsa phetiso ea oksijene. Ke li-oxygenase tsa ho qetela 'me li hloka heme ho kenya tšebetsong oksijene.

Cytochrome P450 monooxygenase e susumetsa ho kenngwa ha oksijene, ho kenngwa ha oksijene le ho kenngwa ha hydroxylation ya alkyl, ho kenngwa ha hydroxylation le ho kenngwa ha ammonia, ho kenngwa ha sebabole, dealkylation (oksijene, sebabole, ammonia), ho kenngwa ha oksijene ka oxidative, ho ntshwa ha oksijene le ho ntshwa ha halogenation.
Ho na le mefuta e 8 ea lihlahisoa tsa cytochrome P450 monooxygenase (Nomoro e le ES-CYP-101～ES-CYP-108) e ntlafalitsoeng ke SyncoZymes.

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Imeile:lchen@syncozymes.com

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Qaqiso ea Sehlahisoa

Li-tag tsa Sehlahisoa

Mabapi le CYP:

Mofuta oa karabelo ea catalytic:

Tlhahisoleseding ea Sehlahisoa:

Li-enzyme	Sete ea ho Hlahlobisisa (SynKit)	Tlhaloso
Phofo ea Enzyme	ES-CYP-101~ ES-CYP-108	sete ea li-monooxygenase tse 8 tsa Cytochrome P450, 50 mg ka 'ngoe lintho tse 8 * 50mg / ntho, kapa bongata bo bong
Sete ea ho Hlahlobisisa (SynKit)	ES-CYP-800	sete ea li-monooxygenase tse 8 tsa Cytochrome P450, 1mg ka 'ngoe Lintho tse 8 * 1mg / ntho

Melemo:

★ Sekhahla se sephara sa substrate.
★ Phetoho e phahameng.
★ Lihlahisoa tse fokolang tse setseng.
★ Maemo a bobebe a karabelo.
★ E sireletsa tikoloho.

Litaelo tsa tšebeliso:

➢ Ka tlwaelo, sistimi ya karabelo e lokela ho kenyeletsa substrate, tharollo ya buffer (Phello e ntle ya pH ya enzyme), coenzyme (NAD(H) kapa NADP(H)), sistimi ya tsosoloso ya coenzyme (mohlala, glucose le glucose dehydrogenase) le ES-CYP. sistimi ya tsosoloso ya coenzyme le coenzyme e ka nkeloa sebaka ke hydrogen peroxide tsamaisong ya karabelo e sa fellang.
➢ Mefuta eohle ea ES-CYPs e tsamaellanang le maemo a fapaneng a karabelo e ntle e lokela ho ithutoa ka bonngoe.
➢ Karoloana e phahameng ea lintho tse ka sebelisoang e ka sitisa ts'ebetso ea ES-CYP. Leha ho le joalo, thibelo e ka fokotsoa ka ho eketsoa ha karoloana e itseng.

Mehlala ea Ts'ebeliso:

Mohlala oa 1⁽¹⁾:

Mohlala oa 2⁽²⁾:

Mohlala oa 3⁽³⁾:

Mohlala oa 4⁽⁴⁾:

Polokelo:

Boloka lilemo tse 2 ka tlase ho -20℃.

Tlhokomeliso:

Le ka mohla u se ke ua kopana le maemo a feteletseng a kang: mocheso o phahameng, pH e phahameng/e tlase le motsoako o nang le manyolo o nang le mahloriso a mangata.

Litšupiso:

1. Zaretzki J, Matlock M, le Swamidass S JJ Chem. Inf. Model, 2013, 53, 3373–3383.
2. Gannett P M., Kabulski J, Perez F A., e tal. J. Am. Chem. Soc., 2006, 128 (26), 8374-8375.
3. Cryle M J., Matovic N J., le De Voss J J. Org. Lett., 2003, 5 (18), 3341–3344.
4. Kawauchi, H., Sasaki, J., Adachi, T., e tal. Biochim. Lithuto tsa bophelo. Acta, 1994, 1219, 179.
5. Yasutake, Y., Fujii, Y.; Cheon, WK e tal. Acta Crystallog. 2009, 65, 372.