CALB e sa sisinyeheng
CALB e sitisoa ke ho monngoa ha resin e sa hlokeng metsi haholo e leng polymer e nang le masoba a mangata, styrene/methacrylate. CALB e sa sitisoang e loketse ho sebelisoa litharollong tsa tlhaho le litsamaisong tse se nang litharollo, 'me e ka sebelisoa hape le ho sebelisoa hape ka makhetlo a mangata maemong a loketseng.
Khoutu ea Sehlahisoa: SZ-CALB- IMMO100A, SZ-CALB- IMMO100B.
★Mosebetsi o phahameng, khetho e phahameng ea chiral le botsitso bo phahameng.
★Ts'ebetso e betere mekhahlelong e seng ea metsi.
★Tlosa habonolo tsamaisong ea karabelo, felisa liketso kapele, 'me u qobe masala a protheine sehlahisoa.
★E ka sebelisoa hape le ho sebelisoa hape ho fokotsa litšenyehelo.
| Mosebetsi | ≥10000PLU/g |
| mefuta ea pH bakeng sa karabelo | 5-9 |
| Tekanyo ea mocheso bakeng sa karabelo | 10-60℃ |
| Ponahalo | CALB-IMMO100-A: Bosootho bo bosehla bo bobebe ho isa ho bosootho CALB-IMMO100-B: E tšoeu ho isa ho sootho bo bobebe |
| Boholo ba likaroloana | 300-500μm |
| Tahlehelo ha omisa ka 105℃ | 0.5%-3.0% |
| Resin bakeng sa ho thibela ho sisinyeha | Polima e nang le masoba a maholo, styrene/methacrylate |
| Tharollo ea karabelo | Metsi, solvent ea tlhaho, jj., kapa ntle le solvent. Bakeng sa karabelo ho li-solvent tse ling tsa tlhaho, metsi a 3% a ka eketsoa ho ntlafatsa phello ea karabelo |
| Boholo ba likaroloana | CALB-IMMO100-A: 200-800 μm CALB-IMMO100-B: 400-1200 μm |
Tlhaloso ea yuniti: Yuniti e le 1 e tsamaellana le tlhahiso ea 1μmol ka motsotso ea propyl laurate ho tsoa ho lauric acid le 1-propanol ho 60℃. CALB-IMMP100-A le CALB-IMMO100-B tse ka holimo li tsamaellana le bajari ba sa sisinyeheng ba nang le boholo bo fapaneng ba likaroloana.
1. Mofuta oa reactor
Enzyme e sa sisinyeheng e sebetsa ho reactor ea kettle batch le ho reactor e tsitsitseng ea phallo e tsoelang pele ea bethe. E lokela ho hlokomeloa ho qoba ho sila ka lebaka la matla a kantle nakong ea ho fepa kapa ho tlatsa.
2. Karabelo pH, mocheso le solvent
Enzyme e sa sisinyeheng e lokela ho eketsoa qetellong, kamora hore thepa e meng e eketsoe le ho qhibilihisoa, mme pH e lokisoe.
Haeba tšebeliso ea substrate kapa ho thehoa ha sehlahisoa ho tla lebisa phetohong ea pH nakong ea karabelo, buffer e lekaneng e lokela ho eketsoa tsamaisong ea karabelo, kapa pH e lokela ho beoa leihlo le ho lokisoa nakong ea karabelo.
Ka har'a mefuta ea CALB ea mamello ea mocheso (ka tlase ho 60 ℃), sekhahla sa phetoho se eketsehile ka keketseho ea mocheso. Ha e sebelisoa ka nepo, mocheso oa karabelo o lokela ho khethoa ho latela botsitso ba substrate kapa sehlahisoa.
Ka kakaretso, karabelo ea hydrolysis ea ester e loketse tsamaisong ea mokhahlelo oa metsi, ha karabelo ea synthesis ea ester e loketse tsamaisong ea mokhahlelo oa lintho tse phelang. Solvent ea lintho tse phelang e ka ba ethanol, tetrahydrofuran, n-hexane, n-heptane le toluene, kapa solvent e tsoakiloeng e loketseng. Bakeng sa karabelo ho li-solvent tse ling tsa lintho tse phelang, metsi a 3% a ka eketsoa ho ntlafatsa phello ea karabelo.
3. Tšebeliso hape le nako ea tšebeletso ea CALB
Tlas'a boemo bo loketseng ba karabelo, CALB e ka fumanoa hape le ho sebelisoa hape, 'me linako tse ikhethileng tsa ts'ebeliso lia fapana ho latela merero e fapaneng.
Haeba CALB e fumanweng e sa sebelisoe hape ka ho sa feleng mme e hloka ho bolokoa kamora ho fumanwa hape, e hloka ho hlatsuoa le ho omiswa le ho kwalwa mochesong wa 2-8 ℃.
Kamora makhetlo a 'maloa a ho sebelisoa hape, haeba katleho ea karabelo e fokotsehile hanyane, CALB e ka eketsoa ka nepo 'me ea tsoela pele ho sebelisoa. Haeba katleho ea karabelo e fokotsehile haholo, e hloka ho nkeloa sebaka.
Mohlala oa 1 (Aminolysis)(1):
Mohlala oa 2 (Aminolysis)(2):
Mohlala oa 3 (Ho kopanngoa ha polyester e bulang lesale)(3):
Mohlala oa 4 (Transesterification, regioselective ea sehlopha sa hydroxyl)(4):
Mohlala oa 5 (Phetoho ea liester, qeto ea kinetic ea alcohols ea racemic)(5):
Mohlala oa 6 (Esterification, qeto ea kinetic ea asiti ea carboxylic)(6):
Mohlala oa 7 (Esterolysis, tharollo ea kinetic)(7):
Mohlala oa 8 (Hydrolysis ea li-amides)(8):
Mohlala oa 9 (Acylation ea li-amine)(9):
Mohlala oa 10 (karabelo ea ho eketsa Aza-Michael)(10):
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