Amidase (AMD)
Li-Enzyme:Ke macromolecular biological catalysts, li-enzyme tse ngata ke liprotheine
Amidase:Catalyze hydrolysis ea mefuta e fapaneng ea li-aliphatic le li-amide tse nkhang hamonate tsa kantle ho naha ka ho fetisetsa sehlopha sa acyl metsing ka tlhahiso ea li-acid tse sa lefelloeng le ammonia.Hydroxamic acids le tse ling tsa organic acid li sebelisoa haholo joalo ka lithethefatsi hobane ke likarolo tsa kholo, lithibela-mafu le li-tumor inhibitors.Li-amidase li ka aroloa ka mofuta oa R le mofuta oa S acylases ho latela catalyst stereoelectivity.
Ntle le ho hlohlelletsa hydrolysis ea li-amides, amidase e ka boela ea susumetsa karabelo ea phetiso ea acyl ka boteng ba li-co-substrates joalo ka hydroxylamine.
Amidase e nang le mehloli e fapaneng e na le likarolo tse fapaneng tsa substrate, tse ling tsa tsona li ka etsa hydrolyze li-amide tse nkhang hamonate, tse ling tsa tsona li ka etsa hydrolyze aliphatic amides, le tse ling tsa hydrolyze α-kapa ω-amino amides.Boholo ba li-amine li na le ts'ebetso e ntle ea catalytic feela bakeng sa li-acyclic kapa li-amide tse nkhang hamonate tse bonolo, empa bakeng sa li-aromatics tse rarahaneng, li-amide tsa heterocyclic, haholo-holo li-amide tse nang le li-ortho substituents, ka kakaretso li na le ts'ebetso e tlase (ke li-enzyme tse seng kae feela tse bonts'ang litlamorao tse ntle tsa catalytic).
Mochine oa Catalytic:
| Li-enzyme | Khoutu ea Sehlahisoa | Khoutu ea Sehlahisoa |
| Phofo ea enzyme | ES-AMD-101~ ES-AMD-119 | sehlopha sa li-amidase tse 19, 50 mg ka 'ngoe lintho tse 19 * 50mg / ntho, kapa bongata bo bong |
| Skrine Kit (SynKit) | ES-AMD-1900 | sete ea 19 amidases, 1 mg ka 'ngoe 19 lintho * 1mg / ntho |
★ Boemo bo phahameng ba substrate.
★ Khetho e matla ea chiral.
★ Ho sebetsa ka mokhoa o phahameng oa phetoho.
★ Lihlahisoa tse fokolang.
★ Maemo a bonolo a karabelo.
★ Ho baballa tikoloho.
➢ Tlhahlobo ea li-enzyme e lokela ho etsoa bakeng sa li-substrate tse khethehileng ka lebaka la sebopeho sa substrate, 'me u fumane enzyme e susumetsang karoloana e lebisitsoeng e nang le phello e ntle ka ho fetisisa.
➢ Le ka mohla u se ke ua kopana le maemo a feteletseng joalo ka: mocheso o phahameng, pH e phahameng/tlase le sehlapolli sa tlhaho se nang le mahloriso a holimo.
➢ Ka tloaelo, sistimi ea karabelo e tlameha ho kenyelletsa substrate, buffer solution (The optimum reaction pH of enzyme).Li-co-substrates tse kang hydroxylamine li lokela ho ba teng tsamaisong ea phetisetso ea acyl.
➢ AMD e tlameha ho kenyelletsoa ho qetela tsamaisong ea karabelo e nang le pH le mocheso o nepahetseng.
➢ Mefuta eohle ea AMD e na le maemo a fapaneng a karabelo, kahoo e 'ngoe le e 'ngoe ea tsona e lokela ho ithutoa ka bo eona.
Mohlala 1(1):
Ts'ebetso ea Hydrolysis ho li-substrates tse fapaneng tsa Amide
| Substrate | Ts'ebetso e khethehileng μmols min-1mg-1 | Substrate | Ts'ebetso e khethehileng μmols min-1mg-1 |
| Acetamide | 3.8 | ο-OH benzamide | 1.4 |
| Propionamide | 3.9 | p-OH benzamide | 1.2 |
| Lactamide | 12.8 | ο-NH2benzamide | 1.0 |
| Butyramide | 11.9 | p-NH2benzamide | 0.8 |
| Isobutyramide | 26.2 | ο- Toluamide | 0.3 |
| Pentanamide | 22.0 | p- Toluamide | 8.1 |
| Hexanamide | 6.4 | Nicotinamide | 1.7 |
| Cyclohexanamide | 19.5 | Isonicotinamide | 1.8 |
| Acrylamide | 10.2 | "Picolinamide" | 2.1 |
| Metacrylamide | 3.5 | 3-Phenylpropionamide | 7.6 |
| Prolinamide | 3.4 | Indol-3-acetamide | 1.9 |
| Benzamide | 6.8 |
Karabelo e entsoe ka 50mM sodium phosphate buffer solution, pH 7.5, ho 70 ℃.
| Amides | Hydroxylamine | Hydrazine |
| Acetamide | 8.4 | 1.4 |
| Propionamide | 18.4 | 3.0 |
| Isobutyramide | 25.0 | 22.7 |
| Benzamide | 9.2 | 6.1 |
Karabelo e entsoe ka 50mM sodium phosphate buffer solution, pH 7.5, ho 70 ℃.
Related reagent concentration: amides, 100 mM(benzamide, 10 mM);hydroxylamine le hydrazine, 400 mM;enzyme 0.9 μM.
Mohlala 2(2):
Mohlala 3(3):
1. D'Abusco AS, Ammendola S., le ba bang.Extremophiles, 2001, 5:183-192.
2. Guo FM, Wu JP, Yang LR, et al.Ts'ebetso ea Biochemistry, 2015, 50 (8): 1400-1404.
3. Zheng RC, Jin JQ, Wu ZM, et al.Bioorganic Chemistry, 2017, E fumaneha inthaneteng 7.
