Nucloside phosphoryalse (NP)

Tlhaloso e Khutšoanyane:

Mabapi le nucleoside phosphoryalse

Ho na le mefuta e 3 ea lihlahisoa tsa NP enzyme (Palo e le ES-NP-101~ ES-NP-103) e ntlafalitsoeng ke SyncoZymes.ES-NP-101 ke purine nucleoside phosphorylase, ES-NP-102 le ES-NP-103 ke pyrimidine nucleoside phosphorylase.Nucleosidephosphorylase e ka senya nucleosides hore e be metheo le pentose phosphate.Nucleoside phosphorylase e ka aroloa ka purine nucleoside phosphorylase le pyrimidine nucleoside phosphorylase ho latela khetho ea metheo ea nucleoside.Purine nucleoside phosphorylase e ka fetola adenosine ho adenine, inosine ho hypoxanthine, guanosine ho guanine, le ho hlahisa ribose phosphate ka nako e le 'ngoe.Pyrimidine nucleoside phosphorylase e ka senya uridine ho uracil mme ea hlahisa ribose phosphate.

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Lintlha tsa Sehlahisoa

Li-tag tsa Sehlahisoa

Mofuta oa karabelo ea Nucloside phosphoryalse:

Lintlha tsa Sehlahisoa:

Li-enzyme	Khoutu ea Sehlahisoa	Tlhaloso
Phofo ea enzyme	ES-NP-101~ ES-NP-103	sete ea 3 Nuclosidephosphoryalse, 50 mg ka 'ngoe lintho tse 3 * 50mg / ntho, kapa bongata bo bong

Mokhoa oa ho hlahloba karabelo ea Nucloside phosphoryalse:

Mokhoa o latelang o ka sebelisoa bakeng sa litšupiso:
1-10 mg / ml Substrate
10 mg / ml enzyme
100 mM Phosphate buffer (pH7.0)
Eketsa karabelo ka mocheso o sa fetoheng (mohlala, 30oC, 150rpm) ka lihora tse 12 ~ 24.Hlakola motsoako o mong le o mong ho theola protheine, tsona tse matlahali li ka sebelisoa bakeng sa tlhahlobo ea TLC, HPLC kapa GC.