Cyclohexanone monooxygenase (CHMO)
Tlhaloso e Khutšoanyane:
Mabapi le Cyclohexanone monooxygenase
ES-CHMO (Cyclohexanone monooxygenase): Baeyer Villiger monooxygenase (BVMO) e nang le cyclohexanone e le substrate, e ka oxidize cyclohexanone ho cyclohexanol e sebelisa oksijene.
Ke mofuta oa enzyme e itšetlehileng ka flavin.Flavin e nang le oxidized e fokotsehile ho flavin ka potoloho ea ho fokotsa coenzyme II (NADPH) bakeng sa oxidation e latelang.Ka hona, cofactor NADPH ea hlokahala potolohong ea flavin.Ho na le mofuta o le mong feela oa sehlahisoa sa enzyme ea CHMO (Palo e le ES-CHMO101) e ntlafalitsoeng ke SyncoZymes, e ka sebelisoang ho oxidize cyclohexanone ho cyclohexanone.
Mofuta oa karabelo ea Catalytic:
Mobile/Wechat/WhatsApp: +86-13681683526
E-mail:lchen@syncozymes.com
| Li-enzyme | Khoutu ea Sehlahisoa | Tlhaloso |
| Skrine Kit (SynKit) | ES-CHMO-101 | sehlopha sa 1 Ketoreductases, 1 mg ka 'ngoe lintho tse 1 * 1mg / ntho |
★ Boemo bo phahameng ba substrate.
★ Khetho e matla ea chiral.
★ Phetoho e phahameng.
★ Lihlahisoa tse fokolang.
★ Maemo a bonolo a karabelo.
★ Ho baballa tikoloho.
➢ Ka tloaelo, sistimi ea karabelo e tlameha ho kenyelletsa substrate, buffer solution, enzyme, coenzyme le coenzyme regeneration system (mohlala, glucose le glucose dehydrogenase).
➢ CHMO e lokela ho kenyelletsoa ho qetela tsamaisong ea karabelo, ka mor'a hore pH le mocheso li lokisoe ho latela boemo ba karabelo.
Mohlala 1(Synthesis of cyclohexanol from cyclohexanol)(1):
Boloka lilemo tse 2 ka tlase ho -20 ℃.
Le ka mohla u se ke ua kopana le maemo a feteletseng a kang: mocheso o phahameng, pH e phahameng / tlase le solvent e phahameng ea organic.
1. Friso, Marco, Appl Microbiol Biotechnol, 2017, 101(20): 7557-7565
