Leucine Dehydrogenase (LeuDH)
Tlhaloso e Khutšoanyane:
Mabapi le Leucine dehydrogenase
Ho na le mofuta o le mong oa sehlahisoa sa enzyme ea LeuDH (Nomoro e le ES-LeuDH-101) e ntlafalitsoeng ke SyncoZymes. LeuDH e ka susumetsa ammoniation e fokotsang ea 4-methylbutanoic acid (letsoai) ho L-leucine kapa oxidation ea leucine ho 4-methylbutanoic acid. ES-LeuDH e ka boela ea susumetsa amination e fokotsang ea α-keto acids e nang le sebopeho se tšoanang ho lokisa li-derivatives tse tsamaellanang tsa amino acid. Ts'ebetsong ea catalysis, NADH ea hlokahala e le transporter ea haeterojene.
Leucine dehydrogenase mofuta oa karabelo ea catalytic
Selefouno/Wechat/WhatsApp: +86-13681683526
Imeile:lchen@syncozymes.com
| Li-enzyme | Khoutu ea Sehlahisoa | Tlhaloso |
| Phofo ea Enzyme | ES-LeuDH-101 | sete ea Monoamine Oxidase e le 1, 50 mg ka 'ngoe Lintho tse 1 * 50mg / ntho, kapa bongata bo bong |
Sistimi e latelang ea karabelo e ka sebelisoa bakeng sa litšupiso:
1-10 mg/ml: Karolo e ka tlase
10 mg/ml: Enzyme
10% (v/v): Cosolvent ea tlhaho (1)
100 mM: Phosphate buffer (pH7.0)
0.2 mg/ml: NAD+
100 mM: Fomate ea Ammonium
10 mg/ml: Formate dehydrogenase (FDH)
Kenya dikarabelo ka sehatsetsing se tsitsitseng sa mocheso (mohlala, 30oC, 150rpm) ka dihora tse 24 ~ 48. Kenya motsoako o mong le o mong ka sehatsetsing ho etsa protheine e potlakileng, mme supernatant e ka sebediswa bakeng sa tlhahlobo ya TLC, HPLC kapa GC.
Tlhokomeliso (1): Sehlahisoa sa tlhaho (joalo ka DMSO, methanol, acetonitrile, IPA, jj.) ke sa boikhethelo, se sebelisetsoa li-substrate tse sa qhibiliheng.
