Leucine Dehydrogenase (LeuDH)
Tlhaloso e Khutšoanyane:
Mabapi le Leucine dehydrogenase
Ho na le mofuta o le mong oa sehlahisoa sa enzyme ea LeuDH (Palo e le ES-LeuDH-101) e entsoeng ke SyncoZymes.LeuDH e ka etsa hore reductive ammoniation ea 4-methylbutanoic acid (letsoai) ho L-leucine kapa oxidation ea leucine ho 4-methylbutanoic acid.ES-LeuDH e ka boela ea etsa hore ho be le phokotso ea amination ea α-keto acid e nang le sebopeho se ts'oanang ho lokisa li-derivatives tsa amino acid.Ts'ebetsong ea catalysis, NADH ea hlokahala joalo ka sepalangoang sa hydrogen.
Leucine dehydrogenasecatalytic reaction mofuta
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E-mail:lchen@syncozymes.com
| Li-enzyme | Khoutu ea Sehlahisoa | Tlhaloso |
| Phofo ea enzyme | ES-LeuDH-101 | sete ea 1 Monoamine Oxidase, 50 mg ntho e 'ngoe le e' ngoe e le 1 * 50mg / ntho, kapa bongata bo bong |
Mokhoa o latelang o ka sebelisoa bakeng sa litšupiso:
1-10 mg / ml: Substrate
10 mg/ml: Enzyme
10% (v/v): Cosolvent ea lintho tse phelang (1)
100 mM: Sekhahla sa Phosphate (pH7.0)
0.2 mg/ml: NAD+
100 mM: Formate ea Ammonium
10 mg/ml: Formate dehydrogenase (FDH)
Eketsa karabelo ka mocheso o sisinyehang (mohlala, 30oC, 150rpm) ka lihora tse 24 ~ 48.Hlakola motsoako o mong le o mong ho theola protheine, tsona tse matlahali li ka sebelisoa bakeng sa tlhahlobo ea TLC, HPLC kapa GC.
Tlhokomeliso (1): Organic solvent (joaloka DMSO, methanol, acetonitrile, IPA, joalo-joalo) ke khetho, e sebelisetsoang li-insoluble substrates.
