Nitrile hydratase (NHT)
Tlhaloso e Khutšoanyane:
Mabapi le ES-NHTs
ES-NHTs: sehlopha sa metalloenzyme, se khethang ho nosetsa li-nitriles ho amide ea mantlha e lumellanang.Ha li sebelisoe haholo bakeng sa ho kopanya li-amino acid, li-amide, li-carboxylic acid le lihlahisoa tsa tsona, empa hape le bakeng sa ho lokisa lithethefatsi tsa chiral.Ho na le mefuta e 24 ea nitrile hydratases e entsoeng ke SyncoZymes (Palo e le ES-NHT-101～ES-NHT-124).
Mofuta oa karabelo ea Catalytic:
Mobile/Wechat/WhatsApp: +86-13681683526
E-mail:lchen@syncozymes.com
| Li-enzyme | Khoutu ea Sehlahisoa | Tlhaloso |
| Phofo ea enzyme | ES-NHT-101~ ES-NHT-124 | sehlopha sa 24 Nitrile Hydratase, 50 mg ka 'ngoe lintho tse 24 * 50mg / ntho, kapa bongata bo bong |
| Skrine Kit (SynKit) | ES-NHT-2400 | sehlopha sa 24 Nnitrile Hydratase, 1 mg ka 'ngoe lintho tse 24 * 1mg / ntho |
★ Boemo bo phahameng ba substrate.
★ Khetho e matla ea chiral.
★ Phetoho e phahameng.
★ Lihlahisoa tse fokolang.
★ Maemo a bonolo a karabelo.
★ Ho baballa tikoloho.
➢ Ka tloaelo, sistimi ea karabelo e tlameha ho kenyelletsa substrate, buffer solution (optimum reaction pH) le ES-NHT.
➢ Li-ES-NHT tsohle li ka lekoa ka ho latellana tsamaisong ea karabelo e kaholimo kapa ka NHT Screening Kit (SynKit NHT).
➢ Mefuta eohle ea li-ES-NHT tse tsamaellanang le maemo a fapaneng a karabelo e nepahetseng li lokela ho ithutoa ka bonngoe.
➢ Sehlahisoa se phahameng kapa sehlahisoa se nang le sona se ka sitisa tšebetso ea ES-NHT.Leha ho le joalo, thibelo e ka imoloha ka ho eketsoa ha batch ea substrate, ho kopana le li-amidase kapa tlhahiso ea lihlahisoa.
Mohlala 1 (ho kopana le amidase)(1):
Mohlala 2(2):
Boloka lilemo tse 2 ka tlase ho -20 ℃.
Le ka mohla u se ke ua kopana le maemo a feteletseng a kang: mocheso o phahameng, pH e phahameng / tlase le solvent e phahameng ea organic.
1 Vojtech V, Ludmila M, Alicja BV, e tal.J Mol Catal B-enzym, 2011, 71: 51-55.
2 Gary W. B, Thomas G, Christopher B. M, e tal.Tetrahedron lett, 2010, 51: 1639-1641.
