Nitrilase (NIT)
Tlhaloso e Khutšoanyane:
Mabapi le Nitrilase
ES-NITs: Ka ho toba fetola sehlopha sa cyanide ho sehlopha sa carboxyl, ho lokisa carboxylic acid, e sebelisoang haholo meriana, ts'ireletso ea tikoloho, indasteri ea lik'hemik'hale, tlatsetso ea lijo, masela, tlhahiso ea temo le masimo a mang.
Ho na le mefuta e 40 ea lihlahisoa tsa Nitrilase(NIT) (Palo e le ES-NIT-101~ES-NIT-140) e ntlafalitsoeng ke SyncoZymes.ES-NIT ke sesebelisoa se sebetsang sa ho etsa tlhahiso ea li-chiral carboxylic acid ka regio- le stereoelective hydrolysis ea mefuta e mengata e fapaneng ea aliphatic le monko o monate.
Mofuta oa karabelo ea Catalytic:
Mobile/Wechat/WhatsApp: +86-13681683526
E-mail:lchen@syncozymes.com
| Li-enzyme | Khoutu ea Sehlahisoa | Tlhaloso |
| Phofo ea enzyme | ES-NIT-101~ ES-NIT-140 | sete ea 40 Nitrilase, 50 mg ka 'ngoe 40 lintho * 50mg / ntho, kapa bongata bo bong |
| Skrine Kit (SynKit) | ES-NIT-4000 | sete ea 40 Nitrilase, 1 mg ka 'ngoe lintho tse 40 * 1mg / ntho |
★ Boemo bo phahameng ba substrate.
★ Phetoho e phahameng.
★ Lihlahisoa tse fokolang.
★ Maemo a bonolo a karabelo.
★ Ho baballa tikoloho.
➢ Ka tloaelo, sistimi ea karabelo e tlameha ho kenyelletsa substrate, buffer solution (optimum reaction pH) le ES-NIT.
➢ Li-ES-NIT tsohle li ka lekoa ka ho latellana tsamaisong ea karabelo e kaholimo kapa ka NIT Screening Kit (SynKit NIT).
➢ Mefuta eohle ea li-ES-NIT tse tsamaellanang le maemo a fapaneng a karabelo e tlamehang ho ithutoa ka bonngoe.
➢ Sehlahisoa se phahameng haholo kapa sehlahisoa se ka sitisang tšebetso ea ES-NIT.Leha ho le joalo, inhibition e ka imoloha ka ho eketsoa ha sehlopha sa substrate.
Mohlala 1(Synthesis of nicotinic acid)(1):
Mohlala 2(Synthesis of (R)-(-)-mandelic acid)(2):
Mohlala 3(Synthesis of baclofen)(3):
Mohlala 4(Synthesis of glycolic acid)(4):
Boloka lilemo tse 2 ka tlase ho -20 ℃.
Le ka mohla u se ke ua kopana le maemo a feteletseng a kang: mocheso o phahameng, pH e phahameng / tlase le solvent e phahameng ea organic.
1 Mathew CD, Nagasawa T, Kobayashi M, e tal.Appl.Tikoloho.Microbiol, 1988, 54(4): 1030.
2 Yamamoto K, Oishi K, Fujimatsu I, e tal.Appl.Tikoloho.Microbiol, 1991, 57(10): 3028.
3 Xu MZ, Ren J, Gong JS, e tal.Chin.J. Biotechnol, 2013, 29(1): 31.
4 Wu S, Fogiel AJ, Petrillo KL, le tal.Biotechnol.Bioeng., 2008, 99(3): 717.
