Threonine deaminase (TDA)

Tlhaloso e Khutšoanyane:

Mabapi le Threonine deaminase

ES-TDA (Threonine deaminase): protheine ea pyridoxal phosphate e susumetsang ho tlosoa ha threonine ho hlahisa 2-ketobutyric acid le ammonia. Pyridoxal 5-phosphate ea hlokahala e le coenzyme tsamaisong ea catalytic. Ho na le mofuta o le mong feela oa sehlahisoa sa enzyme ea TDA (Nomoro e le ES-TDA) e ntlafalitsoeng ke SyncoZymes, e ka sebelisoang ho lokisa 2-ketobutyric acid kapa lihlahisoa tsa eona tse tsoang ho threonine.
Mofuta oa karabelo ea catalytic:

Threonine deaminase TDA2

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Imeile:lchen@syncozymes.com

Romella imeile ho rona

Qaqiso ea Sehlahisoa

Li-tag tsa Sehlahisoa

Tlhahisoleseding ea Sehlahisoa:

Li-enzyme	Khoutu ea Sehlahisoa	Tlhaloso
Phofo ea Enzyme	ES-TDA-101	sete ea 1 Nitrile Reductase, 50 mg ka 'ngoe Lintho tse 1 * 50mg / ntho, kapa bongata bo bong

Melemo:

★ Ho ikhetha ha substrate haholo.
★ Khetho e matla ea chiral.
★ Phetoho e phahameng.
★ Lihlahisoa tse fokolang tse setseng.
★ Maemo a bobebe a karabelo.
★ E sireletsa tikoloho.

Litaelo tsa tšebeliso:

➢ Ka tlwaelo, sistimi ya karabelo e lokela ho kenyeletsa substrate, tharollo ya buffer, enzyme, le coenzyme.
➢ TDA e lokela ho eketsoa qetellong tsamaisong ea karabelo, kamora hore pH le mocheso li lokisoe ho latela boemo ba karabelo.

Mehlala ea Ts'ebeliso:

Mohlala oa 1 (Tlhahiso ea L-2-aminobutyric acid ho tsoa ho threonine)⁽¹⁾:

Polokelo:

Boloka lilemo tse 2 ka tlase ho -20℃.

Tlhokomeliso:

Le ka mohla u se ke ua kopana le maemo a feteletseng a kang: mocheso o phahameng, pH e phahameng/e tlase le motsoako o nang le manyolo o nang le mahloriso a mangata.