Hydroaminase (HAM)
Tlhaloso e Khutšoanyane:
Mabapi le Hydroaminase
Ho na le mefuta e 'meli ea sehlahisoa sa enzyme ea HAM (Nomoro e le ES-HAM-101~ ES-HAM-102) e ntlafalitsoeng ke SyncoZymes. HAM e ka susumetsa ammoniation ea enoic acid kapa li-derivatives tsa eona ho hlahisa chiral amino acid. HAM e ka sebelisoa ho kopanya chiral amino acid (kapa chiral amines) ho tsoa ho enoic acid (kapa alkenes). Mofani oa ammonia, joalo ka metsi a ammonia kapa letsoai la ammonium, oa hlokahala.
Mofuta oa karabelo ea catalytic ea Hydroaminase
Selefouno/Wechat/WhatsApp: +86-13681683526
Imeile:lchen@syncozymes.com
| Li-enzyme | Khoutu ea Sehlahisoa | Tlhaloso |
| Phofo ea Enzyme | ES-HAM-101~ ES-HAM-102 | sete ea 2 Nuclosidephosphoryalse, 50 mg ka 'ngoe 2 lintho * 50mg / ntho, kapa bongata bo bong |
Sistimi e latelang ea karabelo e ka sebelisoa bakeng sa litšupiso:
1-10 mg/ml: Karolo e ka tlase
10 mg/ml: Enzyme
10% (v/v): Cosolvent ea tlhaho (1)
10% (v/v): Ammonia
Fetola pH ho 9.0 pele o eketsa enzyme, 'me enzyme e lokela ho eketsoa qetellong. Kenya liketso ka har'a sesebelisoa se tsitsitseng sa ho shaker mocheso (mohlala, 30oC, 150rpm) ka lihora tse 24-48. Kenya motsoako o mong le o mong ka sehatsetsing ho potlakisa protheine, 'me supernatant e ka sebelisoa bakeng sa tlhahlobo ea TLC, HPLC kapa GC.
Tlhokomeliso (1): Sehlahisoa sa tlhaho (joalo ka DMSO, methanol, acetonitrile, IPA, jj.) ke sa boikhethelo, se sebelisetsoa li-substrate tse sa qhibiliheng.
