Hydroaminase (HAM)
Tlhaloso e Khutšoanyane:
Mabapi le Hydroaminase
Ho na le mefuta e 2 ea sehlahisoa sa enzyme ea HAM (Palo e le ES-HAM-101~ ES-HAM-102) e ntlafalitsoeng ke SyncoZymes.HAM e ka susumetsa ammoniation ea enoic acid kapa lintho tse tsoang ho eona ho hlahisa chiral amino acid.HAM e ka sebelisoa ho kopanya chiral amino acid (kapa chiral amines) ho tloha ho enoic acid (kapa alkenes).Mofani oa ammonia, joalo ka metsi a ammonia kapa letsoai la ammonium, oa hlokahala.
Hydroaminase mofuta oa catalytic reaction
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E-mail:lchen@syncozymes.com
| Li-enzyme | Khoutu ea Sehlahisoa | Tlhaloso |
| Phofo ea enzyme | ES-HAM-101~ ES-HAM-102 | sete ea 2 Nuclosidephosphoryalse, 50 mg ka 'ngoe lintho tse 2 * 50mg / ntho, kapa bongata bo bong |
Mokhoa o latelang o ka sebelisoa bakeng sa litšupiso:
1-10 mg / ml: Substrate
10 mg/ml: Enzyme
10% (v/v): Cosolvent ea lintho tse phelang (1)
10% (v/v): Ammonia
Fetola pH ho 9.0 pele o eketsa enzyme, mme enzyme e lokela ho eketsoa qetellong.Eketsa karabelo ka mocheso o sisinyehang (mohlala, 30oC, 150rpm) ka lihora tse 24 ~ 48.Centrifuge motsoako o mong le o mong ho fokotsa protheine, tsona tse matlahali li ka sebelisoa bakeng sa tlhahlobo ea TLC, HPLC kapa GC.
Tlhokomeliso (1): Organic solvent (joaloka DMSO, methanol, acetonitrile, IPA, joalo-joalo) ke khetho, e sebelisetsoang li-insoluble substrates.
