Ketoreductase (KRED)
KRED ho tsoa ho SyncoZymes: Ho na le mefuta e 191 ea lihlahisoa tsa enzyme ea KRED (Palo e le ES-KRED-101~ES-KRED-291) e ntlafalitsoeng ke SyncoZymes. SZ-KRED e ka sebelisoa haholo phokotsong ea libaka le ea stereoselective ea aldehydes, li-ester tsa beta-ketone, li-ester tsa α-ketone le li-ketone.
Mofuta oa karabelo ea catalytic:
| Li-enzyme | Khoutu ea Sehlahisoa | Tlhaloso |
| Phofo ea Enzyme | Phofo ea Enzyme | sete ea li-Ketoreductase tse 187, 50 mg ka 'ngoe Lintho tse 187 * 50mg / ntho, kapa bongata bo bong |
| Sete ea ho Hlahlobisisa (SynKit) | ES-KRED-18100 | sete ea li-Ketoreductase tse 181, 1 mg ka 'ngoe Lintho tse 181 * 1mg / ntho |
★ Ho ikhetha ha substrate haholo.
★ Khetho e matla ea chiral.
★ Bokgoni bo phahameng ba ho fetolela.
★ Lihlahisoa tse fokolang tse setseng.
★ Maemo a bobebe a karabelo.
★ E sireletsa tikoloho.
➢ Tlhahlobo ea li-enzyme e lokela ho etsoa bakeng sa li-substrate tse itseng ka lebaka la ho khetheha ha substrate, 'me ho fumanoe enzyme e susumetsang substrate e reriloeng ka phello e ntle ka ho fetisisa ea ho susumetsa.
➢ Le ka mohla u se ke ua kopana le maemo a feteletseng a kang: mocheso o phahameng, pH e phahameng/e tlase le motsoako oa tlhaho o nang le mahloriso a phahameng.
➢ Ka tlwaelo, sistimi ya karabelo e lokela ho kenyeletsa substrate, tharollo ya buffer, coenzymes(NAD(H) kapa NADP(H)), sistimi ya tsosoloso ya coenzyme (mohlala, glucose le glucose dehydrogenase).
➢ KRED e lokela ho eketsoa qetellong tsamaisong ea karabelo, kamora hore pH le mocheso li lokisoe ho latela boemo ba karabelo.
➢ Mefuta eohle ea KRED e na le maemo a fapaneng a karabelo a matle, kahoo e 'ngoe le e 'ngoe ea tsona e lokela ho ithutoa ka bonngoe.
Mohlala oa 1(1):
Mohlala oa 2(2):
1. Li Z, Liu WD, Chen X, e tal. Tetrahedron, 2013, 69, 3561-3564.
2. Ni Y, Li CX, Ma HM, e tal. Appl Microbiol Biotechnol, 2011, 89: 1111-1118.
