Lactate Dehydrogenase (LDH)
Tlhaloso e Khutšoanyane:
Mabapi le Lactate dehydrogenase
Ho na le mofuta o le mong oa sehlahisoa sa enzyme ea LDH (Nomoro e le ES-LDH) e ntlafalitsoeng ke SyncoZymes. LDH e ka susumetsa phokotso ea pyruvate ho L-lactic acid, kapa phokotso ea li-α-keto acid tse ling tse tšoanang. Ts'ebetsong ea catalysis, NADH ea hlokahala e le transporter ea haeterojene.
Lactate dehydrogenase mofuta oa karabelo ea catalytic
Selefouno/Wechat/WhatsApp: +86-13681683526
Imeile:lchen@syncozymes.com
| Li-enzyme | Khoutu ea Sehlahisoa | Tlhaloso |
| Phofo ea Enzyme | ES-LDH-101 | sete ea Monoamine Oxidase e le 1, 50 mg ka 'ngoe Lintho tse 1 * 50mg / ntho, kapa bongata bo bong |
Sistimi e latelang ea karabelo e ka sebelisoa bakeng sa litšupiso:
1-10 mg/ml: Karolo e ka tlase
10 mg/ml: Enzyme
10% (v/v): Cosolvent ea tlhaho (1)
100 mM: Phosphate buffer (pH7.0)
0.2 mg/ml: NAD+
100 mM: Fomate ea Ammonium
10 mg/ml: Formate dehydrogenase (FDH)
Kenya dikarabelo ka sehatsetsing se tsitsitseng sa mocheso (mohlala, 30oC, 150rpm) ka dihora tse 24 ~ 48. Kenya motsoako o mong le o mong ka sehatsetsing ho etsa protheine e potlakileng, mme supernatant e ka sebediswa bakeng sa tlhahlobo ya TLC, HPLC kapa GC.
Tlhokomeliso (1): Sehlahisoa sa tlhaho (joalo ka DMSO, methanol, acetonitrile, IPA, jj.) ke sa boikhethelo, se sebelisetsoa li-substrate tse sa qhibiliheng.
