Nitrilase (NIT)
Tlhaloso e Khutšoanyane:
Mabapi le Nitrilase
ES-NITs: Fetola sehlopha sa cyanide ka ho toba ho sehlopha sa carboxyl, ho lokisa asiti ea carboxylic, e sebelisoang haholo meriana, tšireletso ea tikoloho, indasteri ea lik'hemik'hale, lijo tse eketsang, masela, tlhahiso ea temo le masimo a mang.
Ho na le mefuta e 40 ea lihlahisoa tsa Nitrilase (NIT) (Nomoro e le ES-NIT-101~ES-NIT-140) e ntlafalitsoeng ke SyncoZymes. ES-NIT ke sesebelisoa se thusang ho susumetsa tlhahiso ea li-chiral carboxylic acid ka hydrolysis ea regio- le stereoselective ea mefuta e mengata ea li-nitrile tsa aliphatic le aromatic.
Mofuta oa karabelo ea catalytic:
Selefouno/Wechat/WhatsApp: +86-13681683526
Imeile:lchen@syncozymes.com
| Li-enzyme | Khoutu ea Sehlahisoa | Tlhaloso |
| Phofo ea Enzyme | ES-NIT-101~ ES-NIT-140 | sete ea 40 Nitrilase, 50 mg ka 'ngoe Lintho tse 40 * 50mg / ntho, kapa bongata bo bong |
| Sete ea ho Hlahlobisisa (SynKit) | ES-NIT-4000 | sete ea 40 Nitrilase, 1 mg ka 'ngoe Lintho tse 40 * 1mg / ntho |
★ Ho ikhetha ha substrate haholo.
★ Phetoho e phahameng.
★ Lihlahisoa tse fokolang tse setseng.
★ Maemo a bobebe a karabelo.
★ E sireletsa tikoloho.
➢ Ka tlwaelo, sistimi ya karabelo e lokela ho kenyeletsa substrate, tharollo ya buffer (pH e ntle ka ho fetisisa ya karabelo) le ES-NIT.
➢ Li-ES-NIT tsohle li ka lekoa ka ho latellana tsamaisong ea karabelo e kaholimo kapa ka NIT Screening Kit (SynKit NIT).
➢ Mefuta eohle ea ES-NIT e tsamaellanang le maemo a fapaneng a karabelo e ntle e lokela ho ithutoa ka bonngoe.
➢ Karoloana e phahameng ea lintho tse ka sebelisoang e ka sitisa ts'ebetso ea ES-NIT. Leha ho le joalo, thibelo e ka fokotsoa ka ho eketsoa ha karoloana e ka sebelisoang.
Mohlala oa 1 (Tlhahiso ea asiti ea nicotinic)(1):
Mohlala oa 2 (Tlhahiso ea asiti ea (R)-(-)-mandelic)(2):
Mohlala oa 3 (Tlhahiso ea baclofen)(3):
Mohlala oa 4 (Tlhahiso ea glycolic acid)(4):
Boloka lilemo tse 2 ka tlase ho -20℃.
Le ka mohla u se ke ua kopana le maemo a feteletseng a kang: mocheso o phahameng, pH e phahameng/e tlase le motsoako o nang le manyolo o nang le mahloriso a mangata.
1 Mathew CD, Nagasawa T, Kobayashi M, etal. Appl. Environ. Microbiol, 1988, 54(4): 1030.
2 Yamamoto K, Oishi K, Fujimatsu I, e tal. Appl. Tikoloho. Microbiol, 1991, 57(10): 3028.
3 Xu MZ, Ren J, Gong JS, e tal. Chin. J. Biotechnol, 2013, 29(1): 31.
4 Wu S, Fogiel AJ, Petrillo KL, le tal. Biotechnol. Bioeng., 2008, 99(3): 717.
