Nitro reductase (NTR)
Mofuta oa karabelo ea catalytic:
Mokhoa oa ho kenya tšebetsong:
| Li-enzyme | Khoutu ea Sehlahisoa | Tlhaloso |
| Phofo ea Enzyme | ES-NTR-101~ ES-NTR-112 | sete ea li-Nitro Reductase tse 12, 50 mg ka 'ngoe Lintho tse 12 * 50mg / ntho, kapa bongata bo bong |
| Sete ea ho Hlahlobisisa (SynKit) | ES-NTR-1200 | sete ea li-Nitro Reductase tse 12, 1 mg ka 'ngoe Lintho tse 12 * 1mg / ntho |
★ Sekhahla se sephara sa substrate.
★ Phetoho e phahameng.
★ Lihlahisoa tse fokolang tse setseng.
★ Maemo a bobebe a karabelo.
★ E sireletsa tikoloho.
➢ Ka tlwaelo, sistimi ya karabelo e lokela ho kenyeletsa substrate, tharollo ya buffer (pH e nepahetseng ya karabelo), di-coenzyme (NAD(H) kapa NADP(H)), sistimi ya tsosoloso ya di-coenzyme (mohlala, glucose le glucose dehydrogenase) le ES-NTR.
➢ Mefuta eohle ea ES-NTRs e tsamaellanang le maemo a fapaneng a karabelo e ntle e lokela ho ithutoa ka bonngoe.
➢ ES-NTR e lokela ho eketsoa qetellong tsamaisong ea karabelo e nang le pH le mocheso o motle oa karabelo.
Mohlala oa 1(1):
Mohlala oa 2(2):
Boloka lilemo tse 2 ka tlase ho -20℃.
Le ka mohla u se ke ua kopana le maemo a feteletseng a kang: mocheso o phahameng, pH e phahameng/e tlase le motsoako o nang le manyolo o nang le mahloriso a mangata.
1 Dai RJ, Chen J,Lin J, e tal. J Hazard Marer, 2009, 170, 141-143.
2 Betancor L, Berne C, Luckarift H R., e tal. Chem. Commun, 2006, 3640–3642.
