Nitro reductase (NTR)
Mofuta oa karabelo ea Catalytic:
Mochine oa Catalytic:
Li-enzyme | Khoutu ea Sehlahisoa | Tlhaloso |
Phofo ea enzyme | ES-NTR-101~ ES-NTR-112 | sehlopha sa 12 Nitro Reductases, 50 mg ka 'ngoe lintho tse 12 * 50mg / ntho, kapa bongata bo bong |
Skrine Kit (SynKit) | ES-NTR-1200 | sete ea 12 Nitro Reductases, 1 mg ka 'ngoe lintho tse 12 * 1mg / ntho |
★ Maqhubu a pharalletseng a substrate.
★ Phetoho e phahameng.
★ Lihlahisoa tse fokolang.
★ Maemo a bonolo a karabelo.
★ Ho baballa tikoloho.
➢ Ka tloaelo, sistimi ea karabelo e lokela ho kenyelletsa substrate, tharollo ea buffer (optimum reaction pH), coenzymes (NAD(H) kapa NADP(H)), coenzyme regeneration system(mohlala, glucose le glucose dehydrogenase) le ES-NTR.
➢ Mefuta eohle ea li-ES-NTR tse tsamaellanang le maemo a fapaneng a karabelo a nepahetseng li lokela ho ithutoa ka bonngoe.
➢ ES-NTR e tlameha ho kenyelletsoa ho qetela ho sistimi ea karabelo e nang le pH le mocheso o nepahetseng.
Mohlala 1(1):
Mohlala 2(2):
Boloka lilemo tse 2 ka tlase ho -20 ℃.
Le ka mohla u se ke ua kopana le maemo a feteletseng a kang: mocheso o phahameng, pH e phahameng / tlase le solvent e phahameng ea organic.
1 Dai RJ, Chen J,Lin J, e tal.J Hazard Marer, 2009, 170, 141-143.
2 Betancor L, Berne C, Luckarift H R., e tal.Chem.Commun, 2006, 3640–3642.