Alcohol oxidase (AOX)
Tlhaloso e Khutšoanyane:
Mabapi le oxidase ea joala
ES-AOXs: Li-enzyme tsena li khona ho etsa hore oxidation ea lino tse mafura kapa aryl-alcohols li etse aldehydes.Ba sebelisa oksijene ea molek'hule empa ha e hloke cofactor e kentsoeng ka ntle, le hydrogen peroxide e hlahisoa.
Ho na le mefuta e 6 ea lihlahisoa tsa alcohol oxidase (Palo e le ES-AOX-101~ES-AOX-106) e ntlafalitsoeng ke SyncoZymes.ES-AOX101 le ES-AOX102 li khetha li-alphatic alcohol substrates, ES-AOX103~ ES-AOX105 li khetha li-aromatic alcohol substrates, 'me ES-AOX106 ke cholesterol oxidase.SZ-AOX ke sesebelisoa se sebetsang sa ho etsa hore oxidation ea lino tse mafura kapa aryl-alcohols li hlahise aldehydes.
Mofuta oa karabelo ea Catalytic:
Mobile/Wechat/WhatsApp: +86-13681683526
E-mail:lchen@syncozymes.com
| Li-enzyme | Khoutu ea Sehlahisoa | Tlhaloso |
| Phofo ea enzyme | ES-AOX-101~ ES-AOX-105 | sete ea li-oxidase tse 5 tsa joala, 50 mg e 'ngoe le e 'ngoe lintho tse 5 * 50mg / ntho, kapa bongata bo bong. |
| Skrine Kit (SynKit) | ES-AOX-500 | sete ea li-oxidase tse 5 tsa joala, 50 mg e 'ngoe le e 'ngoe lintho tse 5 * 50mg / ntho, kapa bongata bo bong. |
★ Boemo bo phahameng ba substrate.
★ Phetoho e phahameng.
★ Lihlahisoa tse fokolang.
★ Maemo a bonolo a karabelo.
★ Ho baballa tikoloho.
➢ Ka tloaelo, sistimi ea karabelo e tlameha ho kenyelletsa substrate, buffer solution le ES-AOX, mme oksijene e lokela ho ba teng.
➢ Mefuta eohle ea li-ES-AOX tse tsamaellanang le maemo a fapaneng a karabelo, tse ka ithutoang ka bonngoe.
➢ High concentration Substrate kapa sehlahisoa se nang le sona se ka sitisa tšebetso ea ES-AOX.Leha ho le joalo, inhibition e ka imoloha ka ho eketsoa ha sehlopha sa substrate.
➢ Ho bokellana ha H2O2tsamaisong e tla lebisa ho inctivation ea enzyme, e ka rarolloang ka katleho ka tšebeliso ea catalase.
Mohlala 1(Oxidation of aryl-alcohols)(1):
Mohlala 2(Oxidation of fatty alcohols)(2):
Boloka lilemo tse 2 ka tlase ho -20 ℃.
Le ka mohla u se ke ua kopana le maemo a feteletseng a kang: mocheso o phahameng, pH e phahameng / tlase le solvent e phahameng ea organic.
1. Benen J AE, Sa' nchez-Torres P, Wagemaker M JM, e tal.J Biol Chem, 1998, 273 (14): 7865-7872.
2. Mauersberger S, Drechsler H, Oehme G, e tal.Appl Microbiol Biot, 1992, 37: 66-73.
