Phosphokinase (PKase)
Li-enzyme | Khoutu ea Sehlahisoa | Tlhaloso |
Skrine Kit (SynKit) | ES-PKase-101~ES-PKase-121 | sehlopha sa 21 Ketoreductases, 1 mg ka 'ngoe lintho tse 21 * 1mg / ntho |
★ Boemo bo phahameng ba substrate.
★ Khetho e matla ea chiral.
★ Phetoho e phahameng.
★ Lihlahisoa tse fokolang.
★ Maemo a bonolo a karabelo.
★ Ho baballa tikoloho.
➢ Ka tloaelo, sistimi ea karabelo e tlameha ho kenyelletsa substrate, tharollo ea buffer, enzyme, ATP, Mg2+.
➢ PKase e lokela ho ekeletsoa ho qetela tsamaisong ea karabelo, ka mor'a hore pH le mocheso li lokisoe ho latela boemo ba karabelo.
Mohlala 1(Synthesis of nicotinamide ribose phosphate from nicotinamide ribose)(1):
Tlhokomeliso: mehlala ea ts'ebeliso le litšupiso li reretsoe ho bonts'a boholo ba ts'ebeliso ea PKase molemong oa kutloisiso, mme ha e tsamaellane le enzyme e khethehileng ea SyncoZymes.
Boloka lilemo tse 2 ka tlase ho -20 ℃.
Le ka mohla u se ke ua kopana le maemo a feteletseng a kang: mocheso o phahameng, pH e phahameng / tlase le solvent e phahameng ea organic.
1. Khan, Javed A., Song Xiang, le Liang Tong.Sebopeho sa 15.8 (2007): 1005-1013.