Ene reductase (ERED)
Li-ES-ERED li hlahisa mefuta e fapaneng ea li-substrate ka lebaka la mefuta e mengata ea li-substrate.Ka kakaretso, C = C ea α, β -unsaturated metsoako e nang le lihlopha tsa elektronike (ho akarelletsa le ketone aldehyde, lihlopha tsa nitro, carboxylic acids, esters, anhydride, lactones, imines, joalo-joalo) li fokotsoa habonolo ke ES-EREDs, empa the unactivated habeli litlamo ha.
Ho na le mefuta e 46 ea lihlahisoa tsa enzyme ea ERED (Palo e le ES-ERED-101~ES-ERED-146) e entsoeng ke SyncoZymes.
Mochine oa Catalytic:
| Li-enzyme | Khoutu ea Sehlahisoa | Tlhaloso |
| Phofo ea enzyme | ES-ERED-101~ ES-ERED-146 | sete ea 46 Ene Reductases, 50 mg ka 'ngoe 46 lintho * 50mg / ntho, kapa bongata bo bong |
| Skrine Kit (SynKit) | ES-ERED-4600 | sete ea 46 Ene Reductases, 50 mg ka 'ngoe 46 lintho * 50mg / ntho, kapa bongata bo bong |
★ Boemo bo phahameng ba substrate.
★ Khetho e matla ea chiral.
★ Phetoho e phahameng.
★ Lihlahisoa tse fokolang.
★ Maemo a bonolo a karabelo.
★ Ho baballa tikoloho.
★ Tšireletseho e phahameng.
➢ Ka tloaelo, tsamaiso ea karabelo e lokela ho kenyelletsa substrate, tharollo ea buffer (optimum reaction pH), coenzymes(NAD(H) kapa NADP(H) ), coenzyme regeneration system(mohlala, glucose le glucose dehydrogenase) le ES-ERED.
➢ Li-ES-ERED kaofela li ka lekoa ka tatellano ho sistimi ea karabelo e kaholimo kapa ka ERED Screening Kit (SynKit ERED).
➢ Mefuta eohle ea li-ES-ERED tse tsamaellanang le maemo a fapaneng a karabelo a nepahetseng li lokela ho ithutoa ka bo mong.
➢ High concentration Substrate kapa sehlahisoa se nang le sona se ka sitisa tšebetso ea ES-ERED.Leha ho le joalo, inhibition e ka imoloha ka ho eketsoa ha sehlopha sa substrate.
Mohlala 1(α,β-unsaturated aldehydes kapa ketone)(1):
Mohlala 2(α,β-unsaturated carboxylic acid le tse tsoang ho tsona)(2):
Boloka lilemo tse 2 ka tlase ho -20 ℃.
Le ka mohla u se ke ua kopana le maemo a feteletseng a kang: mocheso o phahameng, pH e phahameng / tlase le solvent e phahameng ea organic.
1. Lucídio C, Fardelone J, Augusto R, e tal.J.Mol.Catal.B:Enzym., 2004, 29: 41-45.
2. Stueckler C, Hall M, Ehammer H, e tal..Org.Lett, 2007, 9(26): 5409-5411.
