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Imine reductase (IRED)
Mabapi le Imine reductase
ES-IRED: Sehlopha sa li-enzyme tse etsang hore ho be le phokotso ea C=N bond ho CN bond.Ke ea enzyme ea redox, e nang le coenzyme NADPH bakeng sa lipalangoang tsa haedrojene.Ho ea ka khetho ea chiral ea lihlahisoa, li ka aroloa ka R-IRED le S-IRED.Li-syncozymes li hlahisitse 15 imine reductases (numbIRED ES-IRED-101-ES-IRED-115), e ka sebelisoang haholo phokotsong ea regioselective le stereoselective ea imines ho lokisa chiral amines.
Mofuta oa karabelo ea Catalytic:
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Nitrile reductase (NRED)
Mabapi le Nitrile reductase
Ho na le mofuta oa 3 oa sehlahisoa sa enzyme ea NRED (Palo e le ES-NRED101~ ES-NRED103) e entsoeng ke SyncoZymes.NRED e ka baka phokotso ea sehlopha sa nitrile ho amine.Ts'ebetsong ea catalysis, NADPH ea hlokahala joalo ka sepalangoang sa haedrojene.
Mofuta oa karabelo ea Nitrile reductasecatalytic
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E-mail:lchen@syncozymes.com
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Phenylalanine dehydrogenase (PDH)
Mabapi le Phenylalanine dehydrogenase
ES-PDH: Sehlopha sa oxidoreductase.Karabelo e ntle e ka tsosa deamination ea oxidative ea letsoai la L phenylalanine ka pel'a NAD ho hlahisa letsoai la phenylpyruvate, 'me karabelo e ka morao e ka baka motsoako oa letsoai la amino acid le tsoang letsoai la phenylpyruvate.Li-syncozyme li hlahisitse lintho tse 8 tsa phenylalanine dehydrogenase (e nang le linomoro tsa ES-PDH-101~ES-PDH-108).ES-PDH e ka sebelisoa ho lokisa letsoai la phenylalanine kapa letsoai le tsamaisanang la amino acid ho tsoa letsoai la phenylpyruvic acid kapa tse tsoang ho tse ling.
Mofuta oa karabelo ea Catalytic:
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E-mail:lchen@syncozymes.com
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Leucine Dehydrogenase (LeuDH)
Mabapi le Leucine dehydrogenase
Ho na le mofuta o le mong oa sehlahisoa sa enzyme ea LeuDH (Palo e le ES-LeuDH-101) e entsoeng ke SyncoZymes.LeuDH e ka etsa hore reductive ammoniation ea 4-methylbutanoic acid (letsoai) ho L-leucine kapa oxidation ea leucine ho 4-methylbutanoic acid.ES-LeuDH e ka boela ea etsa hore ho be le phokotso ea amination ea α-keto acid e nang le sebopeho se ts'oanang ho lokisa li-derivatives tsa amino acid.Ts'ebetsong ea catalysis, NADH ea hlokahala joalo ka sepalangoang sa hydrogen.
Leucine dehydrogenasecatalytic reaction mofuta
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E-mail:lchen@syncozymes.com
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Threonine deaminase (TDA)
Mabapi le Threonine deaminase
ES-TDA (Threonine deaminase): protheine ea pyridoxal phosphate e susumetsang deamination ea threonine ho hlahisa 2-ketobutyric acid le ammonia.Pyridoxal 5-phosphate ea hlokahala joalo ka coenzyme tsamaisong ea catalytic.Ho na le mofuta o le mong feela oa sehlahisoa sa enzyme ea TDA (Palo e le ES-TDA) e ntlafalitsoeng ke SyncoZymes, e ka sebelisoang ho lokisa 2-ketobutyric acid kapa tse nkiloeng ho eona ho tsoa ho threonine.
Mofuta oa karabelo ea Catalytic:
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Amine Dehydrogenase (AmDH)
Mabapi le Amine Dehydrogenase
ES-AmDH (Amine Dehydrogenase): enzyme e ka susumetsang ammoniation ea sehlopha sa carbonyl le ho e fokotsa ho sehlopha sa amino.Ke enzyme e itšetlehileng ka NADH ea coenzyme, 'me NADH e sebetsa e le mochine oa hydrogen.Ho na le mefuta e 4 ea lihlahisoa tsa enzyme tsa AmDH (Palo e le ES-AmDH101～ES-AmDH104) e ntlafalitsoeng ke SyncoZymes, e ka sebelisoang ho regioselective kapa stereoelective reductive amination ea aldehydes le ketone.
Mofuta oa karabelo ea Catalytic:
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Nucloside phosphoryalse (NP)
Mabapi le nucleoside phosphoryalse
Ho na le mefuta e 3 ea lihlahisoa tsa NP enzyme (Palo e le ES-NP-101~ ES-NP-103) e ntlafalitsoeng ke SyncoZymes.ES-NP-101 ke purine nucleoside phosphorylase, ES-NP-102 le ES-NP-103 ke pyrimidine nucleoside phosphorylase.Nucleosidephosphorylase e ka senya nucleosides hore e be metheo le pentose phosphate.Nucleoside phosphorylase e ka aroloa ka purine nucleoside phosphorylase le pyrimidine nucleoside phosphorylase ho latela khetho ea metheo ea nucleoside.Purine nucleoside phosphorylase e ka fetola adenosine ho adenine, inosine ho hypoxanthine, guanosine ho guanine, le ho hlahisa ribose phosphate ka nako e le 'ngoe.Pyrimidine nucleoside phosphorylase e ka senya uridine ho uracil mme ea hlahisa ribose phosphate.
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E-mail:lchen@syncozymes.com
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Hydroaminase (HAM)
Mabapi le Hydroaminase
Ho na le mefuta e 2 ea sehlahisoa sa enzyme ea HAM (Palo e le ES-HAM-101~ ES-HAM-102) e ntlafalitsoeng ke SyncoZymes.HAM e ka susumetsa ammoniation ea enoic acid kapa lintho tse tsoang ho eona ho hlahisa chiral amino acid.HAM e ka sebelisoa ho kopanya chiral amino acid (kapa chiral amines) ho tloha ho enoic acid (kapa alkenes).Mofani oa ammonia, joalo ka metsi a ammonia kapa letsoai la ammonium, oa hlokahala.
Hydroaminase mofuta oa catalytic reaction
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CALB e sa sisinyeheng
CALB
Recombinant Lipase B ho tsoa ho Candida antarctica (CALB) e hlahisoa ke ho belisoa ka metsing ka Pichia pastoris e fetotsoeng ka liphatsa tsa lefutso.
CALB e ka sebelisoa mokhahlelong oa metsi kapa organic phase catalytic esterification, esterolysis, transesterification, ring opening polyester synthesis, aminolysis, hydrolysis of amides, acylation of amines and addition reaction.
CALB e na le khetho e phahameng ea chiral le khetho ea maemo, kahoo e ka sebelisoa haholo ho sebetsa ha oli, lijo, meriana, litlolo le liindasteri tse ling tsa lik'hemik'hale.
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E-mail:lchen@syncozymes.com
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NADH oxidase (NOX)
Mabapi le NADH oxidase
ES-NOX (NADH oxidase): NOX e etsa hore ho be le oxidation ea NADH ho NAD+, 'me ke ea oxidoreductase.Ts'ebetsong ea oxidation, O2 ea hlokahala joalo ka oxidant, mme e fokotsehile ho H2O kapa H2O2.Ho na le mefuta e 4 ea lihlahisoa tsa enzyme ea NOX (ES-NOX-101~ES-NOX-104) e ntlafalitsoeng ke k'hamphani ea rona, e ka sebelisetsoang ho nchafatsa oxidation ea coenzyme NAD +.
Mofuta oa karabelo ea Catalytic:
or
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E-mail:lchen@syncozymes.com
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Phenylalanine ammonia lyase (PAL)
Mabapi le Phenylalanine ammonia lyase
ES-PAL: Sehlopha sa li-enzyme tse etsang hore ho be le deamination e tobileng ea L-phenylalanine ho transcinnamic acid.Li-syncozymes li hlahisitse lintho tse 10 tsa phenylalanine ammonia lyase (e baloang ES-PAL-101~ES-PAL-110), e ka sebelisoang ho thibela kapa ho fetola maikutlo a phenylalanine le lihlahisoa tsa eona.
Mofuta oa karabelo ea Catalytic:
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Se-Catalase (CAT)
Mabapi le Catalase
ES-CAT (Catalase): e etsa hore ho be le ho senyeha ha H2O2 ka oksijene le metsi.E fumaneha haholo ho mitochondria, endoplasmic reticulum, sebete sa phoofolo le lisele tse khubelu tsa mali, haholo-holo sebeteng se maemong a holimo, e fana ka ts'ebetso ea ts'ireletso ea antioxidant bakeng sa 'mele.Ho biocatalysis, e sebelisoa haholo ho tlosa karabelo ka sehlahisoa sa hydrogen peroxide le ho fokotsa ho thibela le ho se sebetse ha enzyme ka hydrogen peroxide.Ho na le mofuta o le mong feela oa lihlahisoa tsa enzyme ea CAT tse entsoeng ke SyncoZymes (Palo e le ES-CAT).
Mofuta oa karabelo ea Catalytic:
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