Transaldolase (TAL)

Tlhaloso e Khutšoanyane:

Mabapi le Transaldolase

ES-TAL (Transaldolase): haholo-holo e bua ka threonine transaldolases, e ka susumetsang karabelo ea ho kopanya li-derivatives tsa β-hydroxyphenylalanine ka li-derivatives tsa threonine le benzaldehyde. TAL ke enzyme e itšetlehileng ka PLP. Ho na le mofuta o le mong feela oa sehlahisoa sa enzyme ea TAL (Nomoro e le ES-TAL101) e ntlafalitsoeng ke SyncoZymes, e ka sebelisoang ho kopanya β-hydroxyphenylalanine le li-derivatives tsa eona.

Mofuta oa karabelo ea catalytic:

Transaldolase TAL

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Imeile:lchen@syncozymes.com

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Qaqiso ea Sehlahisoa

Li-tag tsa Sehlahisoa

Melemo:

★ Ho ikhetha ha substrate haholo.
★ Khetho e matla ea chiral.
★ Phetoho e phahameng.
★ Lihlahisoa tse fokolang tse setseng.
★ Maemo a bobebe a karabelo.
★ E sireletsa tikoloho.

Litaelo tsa tšebeliso:

➢ Ka tlwaelo, sistimi ya karabelo e lokela ho kenyeletsa substrate, tharollo ya buffer, enzyme, PLP, Mg²⁺.
➢ TAL e lokela ho eketsoa qetellong tsamaisong ea karabelo, kamora hore pH le mocheso li fetoloe ho latela boemo ba karabelo.

Mehlala ea Ts'ebeliso:

Mohlala oa 1 (Tlhahiso ea (2S, 3R)-p-methylsulfonyl phenylserine ho tsoa ho p-methylsulfonyl benzaldehyde le threonine)⁽¹⁾:

Polokelo:

Boloka lilemo tse 2 ka tlase ho -20℃.

Tlhokomeliso:

Le ka mohla u se ke ua kopana le maemo a feteletseng a kang: mocheso o phahameng, pH e phahameng/e tlase le motsoako o nang le manyolo o nang le mahloriso a mangata.