Cyclohexanone monooxygenase (CHMO)
Tlhaloso e Khutšoanyane:
Mabapi le Cyclohexanone monooxygenase
ES-CHMO (Cyclohexanone monooxygenase): Baeyer Villiger monooxygenase (BVMO) e nang le cyclohexanone e le substrate, e ka kopanya cyclohexanone le cyclohexanol ka oksijene.
Ke mofuta oa enzyme e itšetlehileng ka flavin. Flavin e nang le oxidized e fokotsoa ho ba flavin nakong ea potoloho ea ho fokotsa coenzyme II (NADPH) bakeng sa oxidation e latelang. Ka hona, cofactor NADPH ea hlokahala potolohong ea flavin. Ho na le mofuta o le mong feela oa sehlahisoa sa enzyme ea CHMO (Nomoro e le ES-CHMO101) e ntlafalitsoeng ke SyncoZymes, e ka sebelisoang ho oxidation cyclohexanone ho ba cyclohexanone.
Mofuta oa karabelo ea catalytic:
Selefouno/Wechat/WhatsApp: +86-13681683526
Imeile:lchen@syncozymes.com
| Li-enzyme | Khoutu ea Sehlahisoa | Tlhaloso |
| Sete ea ho Hlahlobisisa (SynKit) | ES-CHMO-101 | sete ea li-Ketoreductase tse 1, 1 mg ka 'ngoe Lintho tse 1 * 1mg / ntho |
★ Ho ikhetha ha substrate haholo.
★ Khetho e matla ea chiral.
★ Phetoho e phahameng.
★ Lihlahisoa tse fokolang tse setseng.
★ Maemo a bobebe a karabelo.
★ E sireletsa tikoloho.
➢ Ka tlwaelo, sistimi ya karabelo e lokela ho kenyeletsa substrate, tharollo ya buffer, enzyme, coenzyme le sistimi ya tsosoloso ya coenzyme (mohlala, glucose le glucose dehydrogenase).
➢ CHMO e lokela ho eketsoa qetellong tsamaisong ea karabelo, kamora hore pH le mocheso li lokisoe ho latela boemo ba karabelo.
Mohlala oa 1 (Tlhahiso ea cyclohexanol ho tsoa ho cyclohexanol)(1):
Boloka lilemo tse 2 ka tlase ho -20℃.
Le ka mohla u se ke ua kopana le maemo a feteletseng a kang: mocheso o phahameng, pH e phahameng/e tlase le motsoako o nang le manyolo o nang le mahloriso a mangata.
1. Friso, Marco, Appl Microbiol Biotechnol, 2017, 101(20): 7557-7565
