Ene reductase (ERED)
Li-ES-ERED li susumetsa mefuta e fapaneng ea li-substrate ka lebaka la mefuta e mengata ea li-substrate. Ka kakaretso, C=C ea metsoako ea α, β-unsaturated e nang le lihlopha tse monyang li-electron (ho kenyeletsoa ketone aldehyde, lihlopha tsa nitro, li-carboxylic acid, li-ester, anhydride, li-lactone, li-imine, jj.) li fokotsoa habonolo ke li-ES-ERED, empa litlamo tse habeli tse sa sebetseng ha li joalo.
Ho na le mefuta e 46 ea lihlahisoa tsa enzyme ea ERED (Nomoro e le ES-ERED-101~ES-ERED-146) e ntlafalitsoeng ke SyncoZymes.
Mokhoa oa ho kenya tšebetsong:
| Li-enzyme | Khoutu ea Sehlahisoa | Tlhaloso |
| Phofo ea Enzyme | ES-ERED-101~ ES-ERED-146 | sete ea 46 Ene Reductases, 50 mg ka 'ngoe 46 lintho * 50mg / ntho, kapa bongata bo bong |
| Sete ea ho Hlahlobisisa (SynKit) | ES-ERED-4600 | sete ea 46 Ene Reductases, 50 mg ka 'ngoe 46 lintho * 50mg / ntho, kapa bongata bo bong |
★ Ho ikhetha ha substrate haholo.
★ Khetho e matla ea chiral.
★ Phetoho e phahameng.
★ Lihlahisoa tse fokolang tse setseng.
★ Maemo a bobebe a karabelo.
★ E sireletsa tikoloho.
★ Polokeho e phahameng.
➢ Ka tlwaelo, sistimi ya karabelo e lokela ho kenyeletsa substrate, tharollo ya buffer (pH e nepahetseng ya karabelo), coenzymes(NAD(H) kapa NADP(H)), sistimi ya tsosoloso ya coenzyme (mohlala, glucose le glucose dehydrogenase) le ES-ERED.
➢ Li-ES-ERED tsohle li ka lekoa ka ho latellana tsamaisong ea karabelo e kaholimo kapa ka ERED Screening Kit (SynKit ERED).
➢ Mefuta eohle ea ES-ERED e tsamaellanang le maemo a fapaneng a karabelo e ntle e lokela ho ithutoa ka bonngoe.
➢ Karoloana e phahameng ea metsi kapa sehlahisoa se nang le eona se ka thibela ts'ebetso ea ES-ERED. Leha ho le joalo, thibelo e ka imolloa ka ho eketsoa ha karoloana ea metsi ka bongata.
Mohlala oa 1(α,β-unsaturated aldehydes kapa ketones)(1):
Mohlala oa 2(α,β-unsaturated carboxylic acids le li-derivatives tsa tsona)(2):
Boloka lilemo tse 2 ka tlase ho -20℃.
Le ka mohla u se ke ua kopana le maemo a feteletseng a kang: mocheso o phahameng, pH e phahameng/e tlase le motsoako o nang le manyolo o nang le mahloriso a mangata.
1. Lucídio C, Fardelone J, Augusto R, e tal. J.Mol.Catal.B:Enzym., 2004, 29: 41-45.
2. Stueckler C, Hall M, Ehammer H, le tal. .Org.Lett, 2007, 9(26): 5409-5411.
