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Nucloside phosphoryalse (NP)
Mabapi le nucleoside phosphoryalse
Ho na le mefuta e 3 ea lihlahisoa tsa NP enzyme (Palo e le ES-NP-101~ ES-NP-103) e ntlafalitsoeng ke SyncoZymes.ES-NP-101 ke purine nucleoside phosphorylase, ES-NP-102 le ES-NP-103 ke pyrimidine nucleoside phosphorylase.Nucleosidephosphorylase e ka senya nucleosides hore e be metheo le pentose phosphate.Nucleoside phosphorylase e ka aroloa ka purine nucleoside phosphorylase le pyrimidine nucleoside phosphorylase ho latela khetho ea metheo ea nucleoside.Purine nucleoside phosphorylase e ka fetola adenosine ho adenine, inosine ho hypoxanthine, guanosine ho guanine, le ho hlahisa ribose phosphate ka nako e le 'ngoe.Pyrimidine nucleoside phosphorylase e ka senya uridine ho uracil mme ea hlahisa ribose phosphate.
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Hydroaminase (HAM)
Mabapi le Hydroaminase
Ho na le mefuta e 2 ea sehlahisoa sa enzyme ea HAM (Palo e le ES-HAM-101~ ES-HAM-102) e ntlafalitsoeng ke SyncoZymes.HAM e ka susumetsa ammoniation ea enoic acid kapa lintho tse tsoang ho eona ho hlahisa chiral amino acid.HAM e ka sebelisoa ho kopanya chiral amino acid (kapa chiral amines) ho tloha ho enoic acid (kapa alkenes).Mofani oa ammonia, joalo ka metsi a ammonia kapa letsoai la ammonium, oa hlokahala.
Hydroaminase mofuta oa catalytic reaction
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CALB e sa sisinyeheng
CALB
Recombinant Lipase B ho tsoa ho Candida antarctica (CALB) e hlahisoa ke ho belisoa ka metsing ka Pichia pastoris e fetotsoeng ka liphatsa tsa lefutso.
CALB e ka sebelisoa mokhahlelong oa metsi kapa organic phase catalytic esterification, esterolysis, transesterification, ring opening polyester synthesis, aminolysis, hydrolysis of amides, acylation of amines and addition reaction.
CALB e na le khetho e phahameng ea chiral le khetho ea maemo, kahoo e ka sebelisoa haholo ho sebetsa ha oli, lijo, meriana, litlolo le liindasteri tse ling tsa lik'hemik'hale.
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NADH oxidase (NOX)
Mabapi le NADH oxidase
ES-NOX (NADH oxidase): NOX e etsa hore ho be le oxidation ea NADH ho NAD+, 'me ke ea oxidoreductase.Ts'ebetsong ea oxidation, O2 ea hlokahala joalo ka oxidant, mme e fokotsehile ho H2O kapa H2O2.Ho na le mefuta e 4 ea lihlahisoa tsa enzyme ea NOX (ES-NOX-101~ES-NOX-104) e ntlafalitsoeng ke k'hamphani ea rona, e ka sebelisetsoang ho nchafatsa oxidation ea coenzyme NAD +.
Mofuta oa karabelo ea Catalytic:
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Phenylalanine ammonia lyase (PAL)
Mabapi le Phenylalanine ammonia lyase
ES-PAL: Sehlopha sa li-enzyme tse etsang hore ho be le deamination e tobileng ea L-phenylalanine ho transcinnamic acid.Li-syncozymes li hlahisitse lintho tse 10 tsa phenylalanine ammonia lyase (e baloang ES-PAL-101~ES-PAL-110), e ka sebelisoang ho thibela kapa ho fetola maikutlo a phenylalanine le lihlahisoa tsa eona.
Mofuta oa karabelo ea Catalytic:
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Se-Catalase (CAT)
Mabapi le Catalase
ES-CAT (Catalase): e etsa hore ho be le ho senyeha ha H2O2 ka oksijene le metsi.E fumaneha haholo ho mitochondria, endoplasmic reticulum, sebete sa phoofolo le lisele tse khubelu tsa mali, haholo-holo sebeteng se maemong a holimo, e fana ka ts'ebetso ea ts'ireletso ea antioxidant bakeng sa 'mele.Ho biocatalysis, e sebelisoa haholo ho tlosa karabelo ka sehlahisoa sa hydrogen peroxide le ho fokotsa ho thibela le ho se sebetse ha enzyme ka hydrogen peroxide.Ho na le mofuta o le mong feela oa lihlahisoa tsa enzyme ea CAT tse entsoeng ke SyncoZymes (Palo e le ES-CAT).
Mofuta oa karabelo ea Catalytic:
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Lysine oxidase (LO)
Mabapi le Lysine oxidase
ES-LO (Lysine oxidase): e etsa hore ho be le oxidation ea L-lysine ho 6-amino-2-oxohexanoic acid (kapa lactone ea eona).Ho na le mofuta o le mong feela oa sehlahisoa sa enzyme ea LO (Palo e le ES-LO) e ntlafalitsoeng ke SyncoZymes.
Mofuta oa karabelo ea Catalytic:
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Phosphokinase (PKase)
Mabapi le Phosphokinase
ES-PKase (Phosphokinase): e susumetsa phetiso ea lihlopha tsa phosphate ho ATP ho metsoako e meng.E boetse e susumetsa phetiso ea lihlopha tsa phosphate ho li-nucleosides tse ling tsa triphosphate ka linako tse ling.Li-kinase tse ngata li hloka li-ion tsa divalent tsa tšepe ho kenya letsoho karabelong (ka kakaretso Mg2 +).Ho na le mefuta e 21 ea lihlahisoa tsa enzyme tsa PKase (Palo e le ES-PKase101~ ES-PKase-121) e ntlafalitsoeng ke SyncoZymes.
Mofuta oa karabelo ea Catalytic:
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Cyclooxygenase (COX)
Mabapi le Cyclooxygenase
ES-COX (Cyclooxygenase): e susumetsa phetoho ea li-bond tsa C = C ho epoxide.Ho na le mefuta e 11 ea lihlahisoa tsa enzyme ea COX (Palo e le ES-COX101~ ES-COX-111) e ntlafalitsoeng ke SyncoZymes.Ho latela mekhoa e fapaneng ea catalytic, COX ea SyncoZymes e arotsoe ka haloperoxidase le styrene monooxygenase.ES-COX101, 102, 107-111 ke ea haloperoxidase, ha ES-COX103-106 e le ea styrene monooxygenase.
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Cyclohexanone monooxygenase (CHMO)
Mabapi le Cyclohexanone monooxygenase
ES-CHMO (Cyclohexanone monooxygenase): Baeyer Villiger monooxygenase (BVMO) e nang le cyclohexanone e le substrate, e ka oxidize cyclohexanone ho cyclohexanol e sebelisa oksijene.
Ke mofuta oa enzyme e itšetlehileng ka flavin.Flavin e nang le oxidized e fokotsehile ho flavin ka potoloho ea ho fokotsa coenzyme II (NADPH) bakeng sa oxidation e latelang.Ka hona, cofactor NADPH ea hlokahala potolohong ea flavin.Ho na le mofuta o le mong feela oa sehlahisoa sa enzyme ea CHMO (Palo e le ES-CHMO101) e ntlafalitsoeng ke SyncoZymes, e ka sebelisoang ho oxidize cyclohexanone ho cyclohexanone.
Mofuta oa karabelo ea Catalytic:
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Carboxylic acid reductase (CAR)
Mabapi le Carboxylic acid reductase
ES-CAR (Carboxylic acid reductase): e baka phokotso ea sehlopha sa carboxyl ho sehlopha sa aldehyde.Ts'ebetsong ea catalytic, ts'ebetso ea ATP le coenzyme NADPH lia hlokahala joalo ka lipalangoang tsa haedrojene.Ho na le mefuta e 2 ea lihlahisoa tsa CAR (Palo e le ES-CAR101~ ES-CAR-102) e entsoeng ke SyncoZymes.
Mofuta oa karabelo ea Catalytic:
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Transaldolase (TAL)
Mabapi le Transaldolase
ES-TAL (Transaldolase): haholo-holo e bua ka threonine transaldolases, e ka susumetsang karabelo ea ho kopanya lihlahisoa tsa β-hydroxyphenylalanine ka threonine le benzaldehyde derivatives.TAL ke enzyme e itšetlehileng ka PLP.Ho na le mofuta o le mong feela oa sehlahisoa sa enzyme ea TAL (Palo e le ES-TAL101) e entsoeng ke SyncoZymes, e ka sebelisoang ho kopanya β-hydroxyphenylalanine le lihlahisoa tsa eona.
Mofuta oa karabelo ea Catalytic:
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