Epoxide hydrolase (EH)
Tlhaloso e Khutšoanyane:
Mabapi le epoxide hydrolase
EH ke ether hydrolase e susumetsang hydrolysis ea stereoselective ea li-epoxide tsa racemic ho hlahisa li-epoxide tse 1, 2-diol le tse sebetsang ka optically, tse ka sebelisoang bakeng sa qeto ea chiral ea li-epoxide kapa ho lokisoa ha li-diols tsa chiral. Li sebelisoa haholo merianeng le mafapheng a mang. Ho na le mefuta e 3 ea lihlahisoa tsa epoxide hydrolases (Nomoro e le ES-EH-101～ES-EH-103) e ntlafalitsoeng ke SyncoZymes. ES-EH ke sesebelisoa se thusang ho susumetsa tlhahiso ea li-alcohol tsa chiral ka hydrolysis ea stereoselective ea li-epoxide tsa aliphatic le aromatic.
Mofuta oa karabelo ea catalytic:
Selefouno/Wechat/WhatsApp: +86-13681683526
Imeile:lchen@syncozymes.com
| Li-enzyme | Khoutu ea Sehlahisoa | Tlhaloso |
| Phofo ea Enzyme | ES-EH-101~ ES-EH-103 | sete ea Epoxide Hydrolases tse 3, 50 mg ka 'ngoe Lintho tse 3 * 50mg / ntho, kapa bongata bo bong |
| Sete ea ho Hlahlobisisa (SynKit) | ES-EH-300 | sete ea Epoxide Hydrolases tse 3, 1 mg ka 'ngoe Lintho tse 3 * 1mg / ntho |
★ Ho ikhetha ha substrate haholo.
★ Khetho e matla ea chiral.
★ Phetoho e phahameng.
★ Lihlahisoa tse fokolang tse setseng.
★ Maemo a bobebe a karabelo.
★ E sireletsa tikoloho.
➢ Ka tlwaelo, sistimi ya karabelo e lokela ho kenyeletsa substrate, tharollo ya buffer (pH e ntle ka ho fetisisa ya karabelo) le ES-EH.
➢ Mefuta eohle ea ES-EH e tsamaellanang le maemo a fapaneng a karabelo e ntle e lokela ho ithutoa ka bonngoe.
➢ Karoloana e phahameng ea lintho tse ka sebelisoang e ka sitisa ts'ebetso ea ES-EH. Leha ho le joalo, thibelo e ka fokotsoa ka ho eketsoa ha karoloana e ka sebelisoang.
Mohlala oa 1(1):
Boloka lilemo tse 2 ka tlase ho -20℃.
Le ka mohla u se ke ua kopana le maemo a feteletseng a kang: mocheso o phahameng, pH e phahameng/e tlase le motsoako o nang le manyolo o nang le mahloriso a mangata.
1. Kim YH, Lee I, Choi SH, e tal. J Mol Catal B-Enzym, 2013, 89: 48-51.
