Esterase le Lipase (PLE le CALB)
Ho na le mefuta e 26 ea lihlahisoa tsa enzyme ea PLE (Nomoro e le ES-PLE-101 ~ ES-PLE-126) e ntlafalitsoeng ke SyncoZymes. ES-PLE e ka sebelisoa bakeng sa hydrolysis ea metsoako ea aliphatic le ester, kapa regioselective le stereoselective resolution ho kopanya chiral acid le li-derivatives tsa tsona.
Mofuta oa karabelo ea catalytic:
★ Ho ikhetha ha substrate haholo.
★ Khetho e matla ea chiral.
★ Phetoho e phahameng.
★ Lihlahisoa tse fokolang tse setseng.
★ Maemo a bobebe a karabelo.
★ E sireletsa tikoloho.
➢ Ka tlwaelo, sistimi ya karabelo e lokela ho kenyeletsa substrate, tharollo ya buffer le ES-PLE. Ho esterification ha di-ES-PLE tse ding ho etswa mohatong wa tlhaho.
➢ Mefuta eohle ea ES-PLE e tsamaellanang le maemo a fapaneng a karabelo e ntle e lokela ho ithutoa ka bonngoe.
Mohlala oa 1 (Biosynthesis ea Pregabalin Intermediate)(1):
Mohlala oa 2(2):
Mohlala oa 3(3):
Mohlala oa 4(4):
Boloka lilemo tse 2 ka tlase ho -20℃.
Le ka mohla u se ke ua kopana le maemo a feteletseng a kang: mocheso o phahameng, pH e phahameng/e tlase.
1. Xu FX, Chen SY, Xu G, e tal. Appl. Biotechnol Bioproc E, 1988, 54(4): 1030.
2. Huang, FC, Lee, LF, Mittal, RSD e tal. J. Am. Chem.Soc, 1975, 97, 4144.
3. Kielbasinski, P., Goralczyk, P., Mikolajczyk, M., e tal. Synlett, 1994, 127.
4. Gais, HJ, Griebel, C., Buschmann, H. Tetrahedron:Asymmetry 2000, 11, 917
