Esterase&Lipase (PLE&CALB)
Ho na le mefuta e 26 ea lihlahisoa tsa enzyme ea PLE (Palo e le ES-PLE-101～ES-PLE-126) e ntlafalitsoeng ke SyncoZymes.ES-PLE e ka sebelisoa bakeng sa hydrolysis ea aliphatic le ester metsoako, kapa regioselective le stereoselective resolution ho synthesize chiral acid le derivatives bona.
Mofuta oa karabelo ea Catalytic:
★ Boemo bo phahameng ba substrate.
★ Khetho e matla ea chiral.
★ Phetoho e phahameng.
★ Lihlahisoa tse fokolang.
★ Maemo a bonolo a karabelo.
★ Ho baballa tikoloho.
➢ Ka tloaelo, sistimi ea karabelo e tlameha ho kenyelletsa substrate, buffer solution le ES-PLE.Esterification ea tse ling tsa ES-PLE e etsoa karolong ea manyolo.
➢ Mefuta eohle ea li-ES-PLE tse tsamaellanang le maemo a fapaneng a karabelo e nepahetseng li lokela ho ithutoa ka bonngoe.
Mohlala 1(Biosynthesis ea Pregabalin Intermediate)(1):
Mohlala 2(2):
Mohlala 3(3):
Mohlala 4(4):
Boloka lilemo tse 2 ka tlase ho -20 ℃.
Le ka mohla u se ke ua kopana le maemo a feteletseng a kang: mocheso o phahameng, pH e phahameng / tlaase.
1. Xu FX, Chen SY, Xu G, e tal.Appl.Biotechnol Bioproc E, 1988, 54(4): 1030.
2. Huang, FC, Lee, LF, Mittal, RSD e tal.J. Am.Chem.Soc, 1975, 97, 4144.
3. Kielbasinski, P., Goralczyk, P., Mikolajczyk, M., e tal.Synlett, 1994, 127.
4. Gais, HJ, Griebel, C., Buschmann, H. Tetrahedron:Asymmetry 2000, 11, 917
