Nitrile reductase (NRED)
Tlhaloso e Khutšoanyane:
Mabapi le Nitrile reductase
Ho na le mefuta e 3 ea sehlahisoa sa enzyme ea NRED (Nomoro e le ES-NRED101~ ES-NRED103) e ntlafalitsoeng ke SyncoZymes. NRED e ka susumetsa phokotso ea sehlopha sa nitrile ho ba amine. Ts'ebetsong ea catalysis, NADPH e hlokahala e le transporter ea haeterojene.
Mofuta oa karabelo ea Nitrile reductasecatalytic
Selefouno/Wechat/WhatsApp: +86-13681683526
Imeile:lchen@syncozymes.com
| Li-enzyme | Khoutu ea Sehlahisoa | Tlhaloso |
| Tlhaloso | ES- NRED -101~ ES- NRED -103 | sete ea 3 Nitrile Reductase, 50 mg ka 'ngoe Lintho tse 3 * 50mg / ntho, kapa bongata bo bong |
Sistimi e latelang ea karabelo e ka sebelisoa bakeng sa litšupiso:
Karolo e ka tlase ea 1-10 mg/ml
Enzyme ea 10 mg/ml
10% (v/v) Cosolvent ea tlhaho (1)
Phosphate buffer ea 100 mM (pH7.0)
0.2 mg/ml NADP+
10 mg/ml ea tsoekere
2 mg/ml Glucose dehydrogenase (GDH)
Kenya dikarabelo ka sehatsetsing se tsitsitseng sa mocheso (mohlala, 30oC, 150rpm) ka dihora tse 24 ~ 48. Kenya motsoako o mong le o mong ka sehatsetsing ho etsa protheine e potlakileng, mme supernatant e ka sebediswa bakeng sa tlhahlobo ya TLC, HPLC kapa GC.
Tlhokomeliso (1): Sehlahisoa sa tlhaho (joalo ka DMSO, methanol, acetonitrile, IPA, jj.) ke sa boikhethelo, se sebelisetsoa li-substrate tse sa qhibiliheng.
