Nitrile reductase (NRED)
Tlhaloso e Khutšoanyane:
Mabapi le Nitrile reductase
Ho na le mofuta oa 3 oa sehlahisoa sa enzyme ea NRED (Palo e le ES-NRED101~ ES-NRED103) e entsoeng ke SyncoZymes.NRED e ka baka phokotso ea sehlopha sa nitrile ho amine.Ts'ebetsong ea catalysis, NADPH ea hlokahala joalo ka sepalangoang sa haedrojene.
Mofuta oa karabelo ea Nitrile reductasecatalytic
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E-mail:lchen@syncozymes.com
| Li-enzyme | Khoutu ea Sehlahisoa | Tlhaloso |
| Tlhaloso | ES- NRED -101~ ES- NRED -103 | sete ea 3 Nitrile Reductase, 50 mg ka 'ngoe lintho tse 3 * 50mg / ntho, kapa bongata bo bong |
Mokhoa o latelang o ka sebelisoa bakeng sa litšupiso:
1-10 mg / ml Substrate
10 mg / ml enzyme
10% (v/v) Cosolvent ea lintho tse phelang (1)
100 mM Phosphate buffer (pH7.0)
0.2 mg/ml NADP+
10 mg / ml ea tsoekere
2 mg/ml Glucose dehydrogenase (GDH)
Eketsa karabelo ka mocheso o sisinyehang (mohlala, 30oC, 150rpm) ka lihora tse 24 ~ 48.Centrifuge motsoako o mong le o mong ho fokotsa protheine, tsona tse matlahali li ka sebelisoa bakeng sa tlhahlobo ea TLC, HPLC kapa GC.
Tlhokomeliso (1): Organic solvent (joaloka DMSO, methanol, acetonitrile, IPA, joalo-joalo) ke khetho, e sebelisetsoang li-insoluble substrates.
