Setšoantšo se Hlahisitsoeng sa Nucloside phosphorylse (NP)

Phosphorylse ea Nucloside (NP)

Tlhaloso e Khutšoanyane:

Mabapi le phosphorylse ea nucleoside

Ho na le mefuta e 3 ea lihlahisoa tsa enzyme ea NP (Palo e le ES-NP-101~ ES-NP-103) e ntlafalitsoeng ke SyncoZymes. ES-NP-101 ke purine nucleoside phosphorylase, ES-NP-102 le ES-NP-103 ke pyrimidine nucleoside phosphorylase. Nucleosidephosphorylase e ka qhala li-nucleosides hore e be metheo le pentose phosphate. Nucleoside phosphorylase e ka aroloa ka purine nucleoside phosphorylase le pyrimidine nucleoside phosphorylase ho latela khetho ea metheo ea nucleoside. Purine nucleoside phosphorylase e ka fetola adenosine ho adenine, inosine ho hypoxanthine, guanosine ho guanine, 'me ea hlahisa ribose phosphate ka nako e le' ngoe. Pyrimidine nucleoside phosphorylase e ka fetola uridine ho uracil 'me ea hlahisa ribose phosphate.

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Qaqiso ea Sehlahisoa

Li-tag tsa Sehlahisoa

Mofuta oa karabelo ea phosphorylse ea Nucloside:

Tlhahisoleseding ea Sehlahisoa:

Li-enzyme	Khoutu ea Sehlahisoa	Tlhaloso
Phofo ea Enzyme	ES-NP-101~ ES-NP-103	sete ea 3 Nuclosidephosphoryalse, 50 mg ka 'ngoe 3 lintho * 50mg / ntho, kapa bongata bo bong

Mokhoa oa ho hlahloba liketso tsa Nucloside phosphorylse:

Sistimi e latelang ea karabelo e ka sebelisoa bakeng sa litšupiso:
Karolo e ka tlase ea 1-10 mg/ml
Enzyme ea 10 mg/ml
Phosphate buffer ea 100 mM (pH7.0)
Kenya dikarabelo ka sehatsetsing se tsitsitseng sa mocheso (mohlala, 30oC, 150rpm) ka dihora tse 12 ~ 24. Kenya motsoako o mong le o mong ka sehatsetsing ho etsa protheine e potlakileng, mme supernatant e ka sebediswa bakeng sa tlhahlobo ya TLC, HPLC kapa GC.