Phenylalanine ammonia lyase (PAL)
Tlhaloso e Khutšoanyane:
Mabapi le Phenylalanine ammonia lyase
ES-PAL: Sehlopha sa li-enzyme tse etsang hore ho be le deamination e tobileng ea L-phenylalanine ho transcinnamic acid.Li-syncozyme li hlahisitse lintho tse 10 tsa phenylalanine ammonia lyase (e baloang ES-PAL-101~ES-PAL-110), e ka sebelisoang ho thibela kapa ho fetola maikutlo a phenylalanine le lihlahisoa tsa eona.
Mofuta oa karabelo ea Catalytic:
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E-mail:lchen@syncozymes.com
| Li-enzyme | Khoutu ea Sehlahisoa | Tlhaloso |
| Phofo ea enzyme | ES-PAL-101~ ES-PAL-110 | sete ea 10 L-phenylalanine ammonia-lyase, 50 mg ka 'ngoe lintho tse 10 * 50mg / ntho, kapa bongata bo bong |
★ Boemo bo phahameng ba substrate.
★ Khetho e matla ea chiral.
★ Phetoho e phahameng.
★ Lihlahisoa tse fokolang.
★ Maemo a bonolo a karabelo.
★ Ho baballa tikoloho.
➢ Ka tloaelo, sistimi ea karabelo e tlameha ho kenyelletsa substrate, buffer solution le enzyme.
➢ Mofuta o mong le o mong oa ES-PAL o tsamaellanang le maemo a fapaneng a karabelo, a ka ithutoang ka bonngoe.
➢ ES-PAL e tlameha ho kenyelletsoa ho qetela tsamaisong ea karabelo ho boloka ts'ebetso.
Mohlala 1(Biosynthesis ea trans cinnamic acid ho tloha phenylalanine)(1):
Mohlala 2(Biosynthesis ea phenylalanine derivative from trans-cinnamic derivative)(2):
Boloka lilemo tse 2 ka tlase ho -20 ℃.
Le ka mohla u se ke ua kopana le maemo a feteletseng a kang: mocheso o phahameng, pH e phahameng / tlase le solvent e phahameng ea organic.
1. Zachary Mays, Karishma Mohan,le al.Chem Commun (Camb).2020, 56, 5255-5258.
2. Ian Rowles, Bas Groenendaal,le al. Tetrahedron,2016. 72, 7343-7347.
