SyncoZymes

lihlahisoa

Transaminase (ATA)

Tlhaloso e Khutšoanyane:

Mabapi le transaminase

ATA ho tsoa ho SyncoZymes: Ho na le mefuta e 66 ea lihlahisoa tsa enzyme tsa ATA (Palo e le ES-ATA-101~ES-ATA-166) e ntlafalitsoeng ke SyncoZymes.SZ-ATA ke sesebelisoa se molemo sa ho etsa hore regio- le stereoselective synthesis ea chiral amines, amino acid le lihlahisoa tsa tsona tse tsoang ho mefuta e fapaneng ea aliphatic le monko o monate oa ketoacids, aldehydes, ketones le ketose.

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Lintlha tsa Sehlahisoa

Li-tag tsa Sehlahisoa

Mabapi le transaminase:

Li-Enzyme: Ke li-macromolecular biological catalysts, li-enzyme tse ngata ke liprotheine.

Transaminase: Sehlopha sa li-enzyme tse susumetsang phetiso ea amino lipakeng tsa li-amino acid le li-keto acid.Li-transaminase ke li-enzyme tsa bohlokoa tsa biological synthesis ea asymmetric le tharollo ea morabe ea li-chiral amines.
Aminotransferase e ka aroloa ka lihlopha tse 'nè ho latela tatellano le sebopeho: Ⅰ, Ⅱ, Ⅲ le Ⅳ.ω-aminotransferase ke ea sehlopha sa II sa li-transaminase, tse atisang ho sebelisoa ha ho etsoa li-chiral amines le li-amino acid tseo e seng tsa tlhaho, tse kang β-amino acid.

ω-aminotransferase: Maemong a mangata, ω-transaminase e bua ka sehlopha sa li-enzyme, tse susumetsang maikutlo a ho fetisa ammonia ntle le α-amino acid e le substrate kapa sehlahisoa.

Mochine oa Catalytic:

Transaminase ATA1

Lintlha tsa Sehlahisoa:

Li-enzyme Khoutu ea Sehlahisoa Khoutu ea Sehlahisoa
Phofo ea enzyme ES-ATA-101~ ES-ATA-165 sehlopha sa 65 ω-Transaminase, 50 mg e 'ngoe le e 'ngoe lintho tse 65 * 50mg / ntho, kapa bongata bo bong
Skrine Kit (SynKit) ES-ATA-6500 sehlopha sa 65 ω-Transaminase, 1 mg ka 'ngoe lintho tse 65 * 1mg / ntho

Melemo ea ATA bakeng sa biotransformation:

★ Boemo bo phahameng ba substrate.
★ Khetho e matla ea chiral.
★ Ho sebetsa ka mokhoa o phahameng oa phetoho.
★ Lihlahisoa tse fokolang.
★ Maemo a bonolo a karabelo.
★ Ho baballa tikoloho.

Litaelo tsa tšebeliso:

➢ Tlhahlobo ea li-enzyme e lokela ho etsoa bakeng sa li-substrate tse khethehileng ka lebaka la sebopeho sa substrate, 'me u fumane enzyme e susumetsang karoloana e lebisitsoeng e nang le phello e ntle ka ho fetisisa.
➢ Le ka mohla u se ke ua kopana le maemo a feteletseng joalo ka: mocheso o phahameng, pH e phahameng/tlase le sehlapolli sa tlhaho se nang le mahloriso a holimo.
➢ Ka tloaelo, mokhoa oa ho itšoara o lokela ho kenyelletsa substrate, tharollo ea buffer, amino donor (e kang amino acid le 1-phenyl ethylamine) kapa receptor (e kang keto acids), coenzyme (PLP), cosolvent (e kang DMSO).
➢ ATA e lokela ho kenyelletsoa ho qetela tsamaisong ea karabelo, ka mor'a hore pH le mocheso li lokisoe ho latela boemo ba karabelo.
➢ Mefuta eohle ea ATA e na le maemo a fapaneng a karabelo, kahoo e 'ngoe le e 'ngoe ea tsona e lokela ho ithutoa ka bonngoe.

Mehlala ea Tšebeliso:

Mohlala 1(synthesis ea Sitagliptin, asymmetric synthesis)(1):

Transaminase ATA2

Mohlala 2 (Mexiletine, Motsoako oa tharollo ea kinetic le asymmetric synthesis)(2):

Transaminase ATA3

Litšupiso:

1 Savile CK, Janey JM, Mundorff EC, le ba bang.Saense, 2010, 329(16), 305-309.
2 Koszelewski D, Pressnitz D, Clay D, et al.Mangolo a tlhaho, 2009,11(21):4810-4812.


  • E fetileng:
  • E 'ngoe:

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