Transaminase (ATA)
Li-enzyme: Ke li-catalyst tsa baeloji tsa macromolecular, li-enzyme tse ngata ke liprotheine.
Li-transaminase: Sehlopha sa li-enzyme tse susumetsang li-amino tse fetisang lipakeng tsa li-amino acid le li-keto acid. Li-transaminase ke li-enzyme tsa bohlokoa tsa baeloji tlhahisong e sa lekanang le qeto ea racemic ea li-chiral amines.
Aminotransferase e ka aroloa ka lihlopha tse 'ne ho latela tatellano le sebopeho: Ⅰ, Ⅱ, Ⅲ le Ⅳ. Li-ω-aminotransferase ke tsa li-transaminase tsa sehlopha sa II, tse atisang ho sebelisoa ho lokiseng li-chiral amine le li-amino acid tse seng tsa tlhaho, tse kang li-β-amino acid.
ω-aminotransferases: Maemong a mangata, ω-transaminase e bolela sehlopha sa li-enzyme, e leng karabelo ea phetisetso ea ammonia e bakang khato ntle le α-amino acid e le substrate kapa sehlahisoa.
Mokhoa oa ho kenya tšebetsong:
| Li-enzyme | Khoutu ea Sehlahisoa | Khoutu ea Sehlahisoa |
| Phofo ea Enzyme | ES-ATA-101~ ES-ATA-165 | sete ea 65 ω-Transaminases, 50 mg ka 'ngoe 65 lintho * 50mg / ntho, kapa bongata bo bong |
| Sete ea ho Hlahlobisisa (SynKit) | ES-ATA-6500 | sete ea 65 ω-Transaminases, 1 mg ka 'ngoe Lintho tse 65 * 1mg / ntho |
★ Ho ikhetha ha substrate haholo.
★ Khetho e matla ea chiral.
★ Bokgoni bo phahameng ba ho fetolela.
★ Lihlahisoa tse fokolang tse setseng.
★ Maemo a bobebe a karabelo.
★ E sireletsa tikoloho.
➢ Tlhahlobo ea li-enzyme e lokela ho etsoa bakeng sa li-substrate tse itseng ka lebaka la ho khetheha ha substrate, 'me ho fumanoe enzyme e susumetsang substrate e reriloeng ka phello e ntle ka ho fetisisa ea ho susumetsa.
➢ Le ka mohla u se ke ua kopana le maemo a feteletseng a kang: mocheso o phahameng, pH e phahameng/e tlase le motsoako oa tlhaho o nang le mahloriso a phahameng.
➢ Ka tlwaelo, sistimi ya karabelo e lokela ho kenyeletsa substrate, tharollo ya buffer, amino donor (jwalo ka amino acid le 1-phenyl ethylamine) kapa receptor (jwalo ka keto acids), coenzyme (PLP), cosolvent (jwalo ka DMSO).
➢ ATA e lokela ho eketsoa qetellong tsamaisong ea karabelo, ka mor'a hore pH le mocheso li lokisoe ho latela boemo ba karabelo.
➢ Mefuta eohle ea ATA e na le maemo a fapaneng a karabelo a matle, kahoo e 'ngoe le e 'ngoe ea tsona e lokela ho ithutoa ka bonngoe.
Mohlala oa 1 (tlhahiso ea Sitagliptin, tlhahiso e sa lekanang)(1):
Mohlala oa 2 (Mexiletine, Motsoako oa qeto ea kinetic le synthesis e sa lekanang)(2):
1 Savile CK, Janey JM, Mundorff EC, jj. Science, 2010, 329(16), 305-309.
2 Koszelewski D, Pressnitz D, Clay D, et al. Mangolo a tlhaho, 2009,11(21):4810-4812.
