Oxidase ea joala (AOX)
Tlhaloso e Khutšoanyane:
Mabapi le oxidase ea joala
ES-AOXs: Li-enzyme tsena li khona ho susumetsa oxidation ea mafura a alcohols kapa aryl-alcohols ho etsa aldehydes. Li sebelisa oksijene ea limolek'hule empa ha li hloke cofactor e ekelitsoeng ka ntle, le hydrogen peroxide e hlahisoang.
Ho na le mefuta e 6 ea lihlahisoa tsa oxidase ea joala (Nomoro e le ES-AOX-101~ES-AOX-106) e ntlafalitsoeng ke SyncoZymes. ES-AOX101 le ES-AOX102 li khetha li-substrate tsa alcohol tsa aliphatic, ES-AOX103~ ES-AOX105 li khetha li-substrate tsa alcohol tse nkhang hamonate, 'me ES-AOX106 ke cholesterol oxidase. SZ-AOX ke sesebelisoa se thusang ho potlakisa oxidation ea alcohols tse mafura kapa aryl-alcohols ho hlahisa aldehydes.
Mofuta oa karabelo ea catalytic:
Selefouno/Wechat/WhatsApp: +86-13681683526
Imeile:lchen@syncozymes.com
| Li-enzyme | Khoutu ea Sehlahisoa | Tlhaloso |
| Phofo ea Enzyme | ES-AOX-101~ ES-AOX-105 | sete ea li-oxidase tse 5 tsa joala, 50 mg ka 'ngoe Lintho tse 5 * 50mg / ntho, kapa bongata bo bong |
| Sete ea ho Hlahlobisisa (SynKit) | ES-AOX-500 | sete ea li-oxidase tse 5 tsa joala, 50 mg ka 'ngoe Lintho tse 5 * 50mg / ntho, kapa bongata bo bong |
★ Ho ikhetha ha substrate haholo.
★ Phetoho e phahameng.
★ Lihlahisoa tse fokolang tse setseng.
★ Maemo a bobebe a karabelo.
★ E sireletsa tikoloho.
➢ Ka tlwaelo, sistimi ya karabelo e lokela ho kenyeletsa substrate, tharollo ya buffer le ES-AOX, mme oksijene e lokela ho ba teng.
➢ Mefuta eohle ea ES-AOX e tsamaellanang le maemo a fapaneng a karabelo e ntle, e ka ithutoang ka bonngoe.
➢ Karoloana e phahameng ea lintho tse ka sebelisoang e ka sitisa ts'ebetso ea ES-AOX. Leha ho le joalo, thibelo e ka fokotsoa ka ho eketsoa ha karoloana e ka sebelisoang.
➢ Ho bokellana ha H2O2tsamaisong ho tla lebisa ho se sebetseng ha li-enzyme, e leng se ka rarolloang ka katleho ka ho sebelisa catalase.
Mohlala oa 1 (Ho oxidation ha aryl-alcohols)(1):
Mohlala oa 2 (Ho oxidation ea alcohols tse mafura)(2):
Boloka lilemo tse 2 ka tlase ho -20℃.
Le ka mohla u se ke ua kopana le maemo a feteletseng a kang: mocheso o phahameng, pH e phahameng/e tlase le motsoako o nang le manyolo o nang le mahloriso a mangata.
1. Benen J AE, Sa' nchez-Torres P, Wagemaker M JM, e tal. J Biol Chem, 1998, 273 (14): 7865-7872.
2. Mauersberger S, Drechsler H, Oehme G, e tal. Appl Microbiol Biot, 1992, 37: 66-73.
