Amine Dehydrogenase (AmDH)

Tlhaloso e Khutšoanyane:

Mabapi le Amine Dehydrogenase

ES-AmDH (Amine Dehydrogenase): enzyme e ka susumetsang ammoniation ea sehlopha sa carbonyl le ho e fokotsa ho ba sehlopha sa amino. Ke enzyme e itšetlehileng ka coenzyme NADH, 'me NADH e sebetsa e le transmitter ea hydrogen. Ho na le mefuta e 4 ea lihlahisoa tsa enzyme ea AmDH (Nomoro e le ES-AmDH101 ~ ES-AmDH104) e ntlafalitsoeng ke SyncoZymes, e ka sebelisoang ho amination ea regioselective kapa stereoselective reductive ea aldehydes le ketones.

Mofuta oa karabelo ea catalytic:

Mabapi le Amine Dehydrogenase2

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Imeile:lchen@syncozymes.com

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Qaqiso ea Sehlahisoa

Li-tag tsa Sehlahisoa

Tlhahisoleseding ea Sehlahisoa:

Melemo:

★ Ho ikhetha ha substrate haholo.
★ Khetho e matla ea chiral.
★ Phetoho e phahameng.
★ Lihlahisoa tse fokolang tse setseng.
★ Maemo a bobebe a karabelo.
★ E sireletsa tikoloho.

Litaelo tsa tšebeliso:

➢ Ka tlwaelo, sistimi ya karabelo e lokela ho kenyeletsa substrate, tharollo ya buffer, enzyme, coenzyme le sistimi ya tsosoloso ya coenzyme (mohlala, glucose le glucose dehydrogenase).
➢ AmDH e lokela ho eketsoa qetellong tsamaisong ea karabelo, kamora hore pH le mocheso li lokisoe ho latela boemo ba karabelo.

Mehlala ea Ts'ebeliso:

Mohlala oa 1 (Ho kopanngoa ha li-amine tsa chiral tse tsamaellanang ka ho fokotsa aminone ea alkyl aryl)⁽¹⁾:

Polokelo:

Boloka lilemo tse 2 ka tlase ho -20℃.

Tlhokomeliso:

Le ka mohla u se ke ua kopana le maemo a feteletseng a kang: mocheso o phahameng, pH e phahameng/e tlase le motsoako o nang le manyolo o nang le mahloriso a mangata.