Amine Dehydrogenase (AmDH)
★ Boemo bo phahameng ba substrate.
★ Khetho e matla ea chiral.
★ Phetoho e phahameng.
★ Lihlahisoa tse fokolang.
★ Maemo a bonolo a karabelo.
★ Ho baballa tikoloho.
➢ Ka tloaelo, sistimi ea karabelo e tlameha ho kenyelletsa substrate, buffer solution, enzyme, coenzyme le coenzyme regeneration system (mohlala, glucose le glucose dehydrogenase).
➢ AmDH e lokela ho kenyelletsoa ho qetela tsamaisong ea karabelo, ka mor'a hore pH le mocheso li lokisoe ho latela boemo ba karabelo.
Mohlala 1(Tlhaloso ea li-amine tsa chiral tse tsamaisanang ka ho fokotsa li-alkyl aryl ketones)(1):
Boloka lilemo tse 2 ka tlase ho -20 ℃.
Le ka mohla u se ke ua kopana le maemo a feteletseng a kang: mocheso o phahameng, pH e phahameng / tlase le solvent e phahameng ea organic.
1. Kong W, Liu Y, Huang C, et al.Khatiso ea Machaba ea Angewandte Chemie, 2022: e202202264.