Cyclooxygenase (COX)

Tlhaloso e Khutšoanyane:

Mabapi le Cyclooxygenase

ES-COX (Cyclooxygenase): e susumetsa phetoho ea likhokahano tsa C=C ho epoxide. Ho na le mefuta e 11 ea lihlahisoa tsa enzyme ea COX (Palo e le ES-COX101~ ES-COX-111) e ntlafalitsoeng ke SyncoZymes. Ho latela mokhoa o fapaneng oa ho etsa li-catalytic, COX ea SyncoZymes e arotsoe ka haloperoxidase le styrene monooxygenase. ES-COX101, 102, 107-111 ke ea haloperoxidase, ha ES-COX103-106 e le ea styrene monooxygenase.

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Mabapi le Cyclooxygenase:

Mofuta oa karabelo ea catalytic:

Haloperoxidase:

Styrene monooxygenase:

Tlhahisoleseding ea Sehlahisoa:

Li-enzyme	Khoutu ea Sehlahisoa	Tlhaloso
Phofo ea Enzyme	ES-COX-101~ ES-COX-110	sete ea 10 NADH Cyclooxygenase, 50 mg ka 'ngoe Lintho tse 10 * 50mg / ntho, kapa bongata bo bong

Melemo:

★ Ho ikhetha ha substrate haholo.
★ Khetho e matla ea chiral.
★ Phetoho e phahameng.
★ Lihlahisoa tse fokolang tse setseng.
★ Maemo a bobebe a karabelo.
★ E sireletsa tikoloho.

Litaelo tsa tšebeliso:

➢ Ka tlwaelo, sistimi ya karabelo ya haloperoxidase e kenyeletsa: substrate, buffer, enzyme, H2O2 le halogen ion. Sistimi ya karabelo ya styrene monooxygenase e kenyeletsa substrate, buffer, enzyme, coenzyme le sistimi ya tsosoloso ya coenzyme.
➢ COX e lokela ho eketsoa qetellong tsamaisong ea karabelo, kamora hore pH le mocheso li lokisoe ho latela boemo ba karabelo.

Mehlala ea Ts'ebeliso:

Mohlala oa 1 (Tlhahiso ea li-derivative tsa epoxy styrene ho tsoa ho li-derivative tsa styrene)⁽¹⁾:

Hlokomela: mehlala ea ts'ebeliso le litšupiso li reretsoe ho bontša boholo ba ts'ebeliso ea COX bakeng sa kutloisiso e bonolo, 'me ha li tsamaisane le enzyme e itseng ea SyncoZymes.

Polokelo:

Boloka lilemo tse 2 ka tlase ho -20℃.

Tlhokomeliso:

Le ka mohla u se ke ua kopana le maemo a feteletseng a kang: mocheso o phahameng, pH e phahameng/e tlase le motsoako o nang le manyolo o nang le mahloriso a mangata.