Nitrile hydratase (NHT)
Tlhaloso e Khutšoanyane:
Mabapi le ES-NHTs
ES-NHTs: sehlopha sa metalloenzyme, e khemisang nitriles ka mokhoa o ikhethileng ho amide ea mantlha e tsamaellanang. Li sebelisoa haholo eseng feela bakeng sa ho kopanya li-amino acid, li-amide, li-carboxylic acid le li-derivatives tsa tsona, empa hape le bakeng sa ho lokisa meriana ea chiral. Ho na le mefuta e 24 ea li-hydratase tsa nitrile tse ntlafalitsoeng ke SyncoZymes (Palo e le ES-NHT-101 ~ ES-NHT-124).
Mofuta oa karabelo ea catalytic:
Selefouno/Wechat/WhatsApp: +86-13681683526
Imeile:lchen@syncozymes.com
| Li-enzyme | Khoutu ea Sehlahisoa | Tlhaloso |
| Phofo ea Enzyme | ES-NHT-101~ ES-NHT-124 | sete ea li-Nitrile Hydratase tse 24, 50 mg ka 'ngoe, lintho tse 24 * 50mg / ntho, kapa bongata bo bong |
| Sete ea ho Hlahlobisisa (SynKit) | ES-NHT-2400 | sete ea li-Nnitrile Hydratase tse 24, 1 mg ka 'ngoe Lintho tse 24 * 1mg / ntho |
★ Ho ikhetha ha substrate haholo.
★ Khetho e matla ea chiral.
★ Phetoho e phahameng.
★ Lihlahisoa tse fokolang tse setseng.
★ Maemo a bobebe a karabelo.
★ E sireletsa tikoloho.
➢ Ka tlwaelo, sistimi ya karabelo e lokela ho kenyeletsa substrate, tharollo ya buffer (pH e nepahetseng ya karabelo) le ES-NHT.
➢ Li-ES-NHT tsohle li ka lekoa ka ho latellana tsamaisong ea karabelo e kaholimo kapa ka NHT Screening Kit (SynKit NHT).
➢ Mefuta eohle ea ES-NHTs e tsamaellanang le maemo a fapaneng a karabelo e ntle e lokela ho ithutoa ka bonngoe.
➢ Karolo e phahameng ea lintho tse ka sebelisoang e ka sitisa ts'ebetso ea ES-NHT. Leha ho le joalo, thibelo e ka fokotsoa ka ho eketsoa ha karolo e ka tlaase, ho kopanngoa le li-amidase kapa ho ntšoa ha sehlahisoa.
Mohlala oa 1 (ho hokahana le amidase)(1):
Mohlala oa 2(2):
Boloka lilemo tse 2 ka tlase ho -20℃.
Le ka mohla u se ke ua kopana le maemo a feteletseng a kang: mocheso o phahameng, pH e phahameng/e tlase le motsoako o nang le manyolo o nang le mahloriso a mangata.
1 Vojtech V, Ludmila M, Alicja BV, e tal. J Mol Catal B-enzym, 2011, 71: 51-55.
2 Gary W. B, Thomas G, Christopher B. M, le tal. Tetrahedron lett, 2010, 51: 1639-1641.
