Phenylalanine dehydrogenase (PDH)
Tlhaloso e Khutšoanyane:
Mabapi le Phenylalanine dehydrogenase
ES-PDH: Sehlopha sa oxidoreductase.Karabelo e ntle e ka tsosa deamination ea oxidative ea letsoai la L phenylalanine ka pel'a NAD ho hlahisa letsoai la phenylpyruvate, 'me karabelo e ka morao e ka baka motsoako oa letsoai la amino acid le tsoang letsoai la phenylpyruvate.Li-syncozyme li hlahisitse lintho tse 8 tsa phenylalanine dehydrogenase (e nang le linomoro tsa ES-PDH-101~ES-PDH-108).ES-PDH e ka sebelisoa ho lokisa letsoai la phenylalanine kapa letsoai le tsamaisanang la amino acid ho tsoa letsoai la phenylpyruvic acid kapa tse tsoang ho tse ling.
Mofuta oa karabelo ea Catalytic:
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E-mail:lchen@syncozymes.com
| Li-enzyme | Khoutu ea Sehlahisoa | Tlhaloso |
| Phofo ea enzyme | ES-PDH-101~ ES-PDH-108 | sete ea 8 Nitrile Reductase, 50 mg e 'ngoe le e' ngoe lintho tse 8 * 50mg / ntho, kapa bongata bo bong |
★ Boemo bo phahameng ba substrate.
★ Khetho e matla ea chiral.
★ Phetoho e phahameng.
★ Lihlahisoa tse fokolang.
★ Maemo a bonolo a karabelo.
★ Ho baballa tikoloho.
➢ Ka tloaelo, sistimi ea karabelo e tlameha ho kenyelletsa substrate, tharollo ea buffer, enzyme, coenzyme (NAD(H)), sistimi ea coenzyme regeneration (mohlala, ammonium formate le formate dehydrogenase).
➢ Mofuta o mong le o mong oa ES-PDH o tsamaellanang le maemo a fapaneng a karabelo, a ka ithutoang ka bonngoe ha ho hlokahala.
➢ ES-PDH e tlameha ho kenyelletsoa ho qetela tsamaisong ea karabelo ho boloka ts'ebetso.
Mohlala 1(Biosynthesis ea phenylalanine derivatives ho tsoa ho phenylpyruvate derivatives)(1):
Boloka lilemo tse 2 ka tlase ho -20 ℃.
Le ka mohla u se ke ua kopana le maemo a feteletseng a kang: mocheso o phahameng, pH e phahameng / tlase le solvent e phahameng ea organic.
1. Francesca Paradisi, Stuart Collins, et al.Journal of Biotechnology, 2007, 128, 408-411.
