Phenylalanine dehydrogenase (PDH)
Tlhaloso e Khutšoanyane:
Mabapi le Phenylalanine dehydrogenase
ES-PDH: Sehlopha sa oxidoreductase. Karabelo e ntle e ka susumetsa ho tlosoa ha oxidative ha letsoai la phenylalanine la L ha le le teng ho NAD ho hlahisa letsoai la phenylpyruvate, 'me karabelo e fapaneng e ka susumetsa tlhahiso ea letsoai la amino acid le tsamaellanang le letsoai la phenylpyruvate. Syncozymes e hlahisitse lintho tse 8 tsa phenylalanine dehydrogenase (tse nkiloeng ka nomoro ea ES-PDH-101~ES-PDH-108). ES-PDH e ka sebelisoa ho lokisa letsoai la phenylalanine kapa letsoai la amino acid le tsamaellanang le letsoai la phenylpyruvic acid kapa li-derivatives.
Mofuta oa karabelo ea catalytic:
Selefouno/Wechat/WhatsApp: +86-13681683526
Imeile:lchen@syncozymes.com
| Li-enzyme | Khoutu ea Sehlahisoa | Tlhaloso |
| Phofo ea Enzyme | ES-PDH-101~ ES-PDH-108 | sete ea 8 Nitrile Reductase, 50 mg ka 'ngoe lintho tse 8 * 50mg / ntho, kapa bongata bo bong |
★ Ho ikhetha ha substrate haholo.
★ Khetho e matla ea chiral.
★ Phetoho e phahameng.
★ Lihlahisoa tse fokolang tse setseng.
★ Maemo a bobebe a karabelo.
★ E sireletsa tikoloho.
➢ Ka tlwaelo, sistimi ya karabelo e lokela ho kenyeletsa substrate, tharollo ya buffer, enzyme, coenzyme (NAD(H)), sistimi ya tsosoloso ya coenzyme (mohlala, ammonium formate le formate dehydrogenase).
➢ Mofuta o mong le o mong oa ES-PDH o tsamaellanang le maemo a fapaneng a karabelo a matle ka ho fetisisa, a ka ithutoang ka bonngoe haeba ho hlokahala.
➢ ES-PDH e lokela ho eketsoa qetellong tsamaisong ea karabelo ho boloka ts'ebetso.
Mohlala oa 1 (Biosynthesis ea li-derivatives tsa phenylalanine ho tsoa ho li-derivatives tsa phenylpyruvate)(1):
Boloka lilemo tse 2 ka tlase ho -20℃.
Le ka mohla u se ke ua kopana le maemo a feteletseng a kang: mocheso o phahameng, pH e phahameng/e tlase le motsoako o nang le manyolo o nang le mahloriso a mangata.
1. Francesca Paradisi, Stuart Collins, jj. Koranta ea Biotechnology, 2007, 128, 408–411.
