Benzaldehyde lyase (BAL)

Tlhaloso e Khutšoanyane:

Mabapi le Benzaldehyde lyase

ES-BAL (Benzaldehyde lyase): mofuta oa enzyme e susumetsang hydroxymethylation ea aldehydes e nkhang hamonate le e nang le formaldehyde ho hlahisa α- Hydroxymethyl ketone e tsamaellanang. BAL ke enzyme e itšetlehileng ka thiamine diphosphate (ThDP). Ho na le mofuta o le mong feela oa sehlahisoa sa enzyme ea BAL (Nomoro e le ES-BAL101) e ntlafalitsoeng ke SyncoZymes, e ka sebelisoang bakeng sa hydroxymethylation ea aldehydes e nkhang hamonate le e nang le e.

Mofuta oa karabelo ea catalytic:

Benzaldehyde lyase BAL

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Imeile:lchen@syncozymes.com

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Qaqiso ea Sehlahisoa

Li-tag tsa Sehlahisoa

Melemo:

★ Ho ikhetha ha substrate haholo.
★ Khetho e matla ea chiral.
★ Phetoho e phahameng.
★ Lihlahisoa tse fokolang tse setseng.
★ Maemo a bobebe a karabelo.
★ E sireletsa tikoloho.

Litaelo tsa tšebeliso:

➢ Ka tlwaelo, sistimi ya karabelo e lokela ho kenyeletsa substrate, tharollo ya buffer, enzyme, ThDP, Mg²⁺.
➢ BAL e lokela ho eketsoa qetellong tsamaisong ea karabelo, kamora hore pH le mocheso li lokisoe ho latela boemo ba karabelo.

Mehlala ea Ts'ebeliso:

Mohlala oa 1 (Tlhahiso ea li-amino alcohols tse 1,2 tse nketsoeng sebaka ke N ho tsoa ho aldehydes)⁽¹⁾:

Polokelo:

Boloka lilemo tse 2 ka tlase ho -20℃.

Tlhokomeliso:

Le ka mohla u se ke ua kopana le maemo a feteletseng a kang: mocheso o phahameng, pH e phahameng/e tlase le motsoako o nang le manyolo o nang le mahloriso a mangata.